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Catalogue No.


Cell Line Name


Cell Line Description

A 6-thioguanine-resistant clone of N18 (a clonal line produced from the mouse neuroblastoma C1300). (Mouse strain A/Jax). This cell line exhibits high levels of acetylcholinesterase. Found to be tumourigenic in BALB/c nude mice.



Expresses acetylcholinesterase

Tissue of Origin



mean chromosome number is 89


Used in the generation of cell hybrids



Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine

Growth Mode


Additional Info


Professor Bernd Hamprecht, Interfaculty Institute for Biochemistry, University of Tuebingen, Hoppe-Seyler Street 4, 72076 Tuebingen, Germany.

Country of Origin


Hazard Group (ACDP)

Hazard Group (ACDP) 2



Minna et al., (1972) Genetic dissection of neural properties using somatic cell Hybrids. Nature New Biology 235: 225-231.


Heumann et al., (1977) Tumorigenicity of neuroblastoma x glioma hybrid cells in nude mice and reintroduction of transplanted cells into culture. Eur. J. of Cancer 13: 1417-1420.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: N18TG2 (ECACC 08062523).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.