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MT-4

MT-4

Catalogue No.

08081402

Cell Line Name

MT-4

Cell Line Description

Derived from a 50 year old Japanese male with generalised lymphadenopathy and heptosplenomegaly (Adult T-cell Leukaemia) by co-culture of his peripheral leukocytes with male umbilical cord lymphocytes. The cells carry HTLV-1 and support the growth of HIV. The cells should be handled under laboratory containment level 3 conditions. STR profiling at ECACC confirms the presence of a Y chromosome

Discontinued Message

Pending internal review.

General Info

Species

Human

Release Conditions

Restricted – all customers must complete the specific depositor MTA.

Characteristics

Products

Adult T-cell leukaemia virus (ALT; HTLV-1)

Tissue of Origin

Not specified

Karyotype

2n=46

Applications

MT-4 cells are susceptible to virus infection with HTLV-III and produces viral specific antigens and viral progeny in 4 days.

Disease

Neuroblastoma

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Seed cultures between 2-3x100,000 cells/ml. Saturation density 9 x 100,000 cells/ml; 5% CO₂; 37°C.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Decomplemented Foetal Bovine Serum (FBS).

Growth Mode

Non-adherent or adherent on poly-L-lysine treated plastic

Additional Info

Depositor

Professor Myra McClure, Jefferiss Research Trust Laboratories, Wright-Fleming Institute, Faculty of Medicine, Imperial College, London, St Mary's Campus, Norfolk Place, London W2 1PG

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Harada et al., (1985) Infection of human T-lymphotropic virus type-1 (HTLV-1)-bearing MT-4 cells with HTLV-III (AIDS virus): chronological studies of early events. Virology, 146: 272-281.

Bibliography

Miyoshi et al., (1982) Gann Monographs 28: 770-771.Harada et al., (1985) Infection of HTLV-III/LAV in HTLV-1-carrying Cells MT-2 and MT-4 and Application in a Plaque Assay. Science 229: 563-566.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MT-4 (ECACC 08081402).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.