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Catalogue No.


Cell Line Name


Cell Line Description

From kidney of normal female adult Cocker Spaniel in 1958 by SH Madin and NB Darby (Madin Darby Canine Kidney). Supports growth of wide range of animal viruses: VSV (Indiana strain), infectious Canine Hepatitis, Vaccinia, Coxsackie B5, Adeno and reo viruses, SVEV.


Tissue of Origin



2n = 78


Virus studies: SVEV, VSV, Vaccinia, adeno and reoviruses, Coxsackie B5 et al


None Stated

Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Cells attach firmly and require at least 2 PBS washes prior to addition of trypsin/EDTA.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Dr D Tyrrell, MRC Common Cold Unit, Salisbury

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Proc Soc Exp Biol Med 1958;98:574; Proc Soc Exp Biol Med 1966;122:931


1)Biochem J; 1996 Nov 1: 319 ( Pt 3): 909-12"The lysosomal Ca2+ pool in MDCK cells can be released by ins(1,4,5)P3-dependent hormones or thapsigargin but does not activate store-operated Ca2+ entry."2) J Eukaryot Microbiol; 1996 Sep-Oct: 43(5): 87S"In vitro evaluation of anticryptosporidial agents using MDCK cell culture and chemiluminescence immunoassay."3)Cell Biol Int; 1996 Jul: 20(7): 489-99"Disruption of cell-cell adhesion in the presence of sodium butyrate activates expression of the 92 kDa type IV collagenase in MDCK cells."4)Am J Physiol; 1996 Oct: 271(4 Pt 1): C1064-72"Role of PLA2, PLC, and PLD in bradykinin-induced release of arachidonic acid in MDCK cells."5) Am J Physiol; 1996 Sep: 271(3 Pt 2): F610-8"Heterogeneity of P2u- and P2y-purinergic receptor regulation of phospholipases in MDCK cells."6)Am J Physiol; 1996 Jul: 271(1 Pt 1): C226-34"Apical membrane permeability of MDCK cells."7)Am J Physiol; 1996 Jan: 270(1 Pt 2): F220-8"Expression of proximal tubular Na-Pi and Na-SO4 cotransporters in MDCK and LLC-PK1 cells by transfection."8)Am J Physiol; 1996 Jan: 270(1 Pt 1): G176-83"Polarized GP2 secretion in MDCK cells via GPI targeting and apical membrane-restricted proteolysis."9)Am J Physiol; 1996 Jan: 270(1 Pt 1): C200-7"Betaine and inositol reduce MDCK cell glycerophosphocholine by stimulating its degradation."10)J Membr Biol; 1996 Sep: 153(1): 1-11"Hydraulic properties of MDCK cell epithelium."11)J Membr Biol; 1996 Jan: 149(1): 49-55"Transfection alters ion transport in MDCK cells."12)J Nihon Univ Sch Dent; 1995 Dec: 37(4): 201-8"The influence of scattering on temporo-regional proliferation in the cultured Madin-Darby canine kidney (MDCK) cells."13)J Gen Virol; 1996 Oct: 77 ( Pt 10): 2507-14"Virus entry into a polarized epithelial cell line (MDCK): similarities and dissimilarities between influenza C virus and bovine oronavirus."14)Mol Biol Cell; 1996 Jul: 7(7): 1025-41"Mechanisms of integrin-mediated calcium signaling in MDCK cells: regulation of adhesion by IP3- and store-independent calcium influx."15)Am J Physiol; 1996 Mar: 270(3 Pt 2): F419-24"Hypertonic activation and recovery of system A amino acid transport in renal MDCK cells."16)Pflugers Arch; 1996 Aug: 432(4): 685-91"Characterization of hormone-stimulated Na+ transport in a high-resistance clone of the MDCK cell line."17)J Membr Biol; 1995 Dec: 148(3): 223-32"Determination of the Na permeability of the tight junctions of MDCK cells by fluorescence microscopy."18)J Cell Biol; 1996 Oct: 135(1): 139-52"Apical and basolateral endosomes of MDCK cells are interconnected and contain a polarized sorting mechanism."19)J Cell Biochem; 1995 Dec: 59(4): 453-62"Cyclic-AMP deficient MDCK cells form tubules."20) Scanning Microsc; 1995 Jun: 9(2): 587-96"Alterations in MDCK and LLC-PK1 cells exposed to oxalate and calcium oxalate monohydrate crystals."21)C R Acad Sci III; 1996 Apr: 319(4): 277-87"Method of measurement of cadmium influx by fura-2 titration in MDCK cell evidenced. Fluorescence video-microscopy study"22)Pharmazie; 1996 May: 51(5): 341-5"Phonophoretic permeation of procaine hydrochloride through an MDCK cell monolayer"23)J Virol; 1996 Sep: 70(9): 6508-15"Transmembrane domain of influenza virus neuraminidase, a type II protein, possesses an apical sorting signal in polarized MDCK cells."24) Cell Calcium; 1996 Feb: 19(2): 157-65"The lysosomal compartment as intracellular calcium store in MDCK cells: a possible involvement in InsP3-mediated Ca2+ release."25)J Cell Biol; 1996 Jun: 133(6): 1265-76"Traffic, polarity, and detergent solubility of a glycosylphosphatidylinositol-anchored protein after LDL-deprivation of MDCK cells."26) Arch Virol; 1996: 141(5): 923-33"A variant of MDCK cell line which restricted growth of influenza viruses mainly through suppression of viral primary transcription."27)Int J Urol; 1996 Jan: 3(1): 23-6"Microlith formation in vitro by Madin Darby canine kidney (MDCK) cells."28) Biochem J; 1996 May 1: 315 ( Pt 3): 983-7"Tight connection between choline transport and phosphatidylcholine synthesis in MDCK cells."29) Am J Physiol; 1996 Mar: 270(3 Pt 1): C753-62"Polarity of TRH receptors in transfected MDCK cells is independent of endocytosis signals and G protein coupling."30)Biochim Biophys Acta; 1996 Apr 24: 1311(2): 93-101"The cytosolic glutathione S-transferase isoenzymes in the dog kidney cortex as compared with the corresponding MDCK renal cell line."31)EMBO J; 1996 Apr 1: 15(7): 1471-81"Reconstitution of transcytosis in SLO-permeabilized MDCK cells: existence of an NSF-dependent fusion mechanism with the apical surface of MDCK cells."32)Biophys J; 1995 Dec: 69(6): 2800-7"Impedance analysis of MDCK cells measured by electric cell-substrate impedance sensing."33)Cell Struct Funct; 1995 Oct: 20(5): 387-93"Effects of tyrosine phosphorylation on tight junctions in temperature-sensitive v-src-transfected MDCK cells."34) Chromosoma; 1996: 104(5): 321-31"Cell cycle related behavior of a chromosomal scaffold protein in MDCK epithelial cells."35)Kidney Int; 1995 Oct: 48(4): 1200-5"Properties and regulation of ion channels in MDCK cells."36)Biochem Soc Trans; 1995 Aug: 23(3): 535-8"The role of microtubules in apical and basolateral endocytosis in epithelial Madin-Darby canine kidney (MDCK) cells."37)Epithelial Cell Biol; 1995: 4(1): 17-24"Characterization of Na,K-ATPase isoform expression and activity in MDCK and Caco-2 epithelial cells."38)Antiviral Res; 1995 Aug: 27(4): 425-30"Inhibitory effect of bafilomycin A1, a specific inhibitor of vacuolar-type proton pump, on the growth of influenza A and B viruses in MDCK cells."39)Int J Biochem Cell Biol; 1995 Oct: 27(10): 1055-63"Hyperosmolality stimulates phospholipase A2 activity in rabbit renal medulla and in Madin-Darby canine kidney (MDCK) cells."40)Arch Toxicol; 1995: 69(6): 421-4"Influence of glucose on the toxicity of oxophenylarsine in MDCK cells."41)J Physiol (Lond); 1995 Aug 1: 486 ( Pt 3): 557-69"A highly calcium-selective cation current activated by intracellular calcium release in MDCK cells."42) J Cell Sci; 1995 Aug: 108 ( Pt 8): 2917-26"Phosphorylation of the tight junction protein cingulin and the effects of protein kinase inhibitors and activators in MDCK epithelial cells."43)J Cell Sci; 1995 Aug: 108 ( Pt 8): 2791-800"Galectin-3 expression and effects on cyst enlargement and tubulogenesis in kidney epithelial MDCK cells cultured in three-dimensional matrices in vitro."44)Fundam Appl Toxicol; 1995 Aug: 27(1): 1-8"Reversal of oxophenylarsine-induced inhibition of glucose uptake in MDCK cells"45)FEBS Lett; 1995 Oct 9: 373(2): 123-6"Phosphatase toward MAP kinase is regulated by osmolarity in Madin-Darby canine kidney (MDCK) cells."46)FEMS Microbiol Lett; 1995 Jul 15: 130(1): 45-9"An unstable small-colony variant of a noninvasive mutant of Salmonella typhimurium is highly invasive for MDCK cells."47)Biochim Biophys Acta; 1995 Sep 21: 1268(3): 325-8"Clusterin gene expression in apoptotic MDCK cells is dependent on the apoptosis-inducing stimulus."48)Biochim Biophys Acta; 1995 Sep 14: 1258(2): 206-14"Regulation of bradykinin-stimulated phospholipase C and arachidonic acid release by protein kinase A in MDCK-D1 cells."49)Pflugers Arch; 1995 Apr: 429(6): 832-40"Mechanisms of dopamine effects on Na-K-ATPase activity in Madin-Darby canine kidney (MDCK) epithelial cells."50)J Membr Biol; 1995 Mar: 144(1): 21-30"The chloride concentration in the lateral intercellular spaces of MDCK cell monolayers."

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: MDCK (ECACC 84121903).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.