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L35

L35

Catalogue No.

91012319

Cell Line Name

L35

Cell Line Description

One of a series of cell lines established from mononuclear cells of a boar (Sus scrofa domestica L) which was injected with Shimozuma cells producing porcine retrovirus (Tsukuba-1). The cells express MSA4. The cells contain retrovirus particles and are positive in RT assay.

Characteristics

Tissue of Origin

Blood

Karyotype

Modal no. 36.5

Applications

RT assay and virus studies: Porcine retrovirus

Disease

None Stated

Culture Conditions

Subculture Routine

Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO₂; 37°C. Cells grow as small clumps.

Culture Medium

RPMI 1640 + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr B Kaeffer, INRA, Laboratoire Pathologie, Nouzilly

Country of Origin

France

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Kaeffer B, Bottreau E, Phan Thanh L, Olivier M, Salmon H.1990 Histocompatible miniature, boar model: selection of transformed cell lines of B and T lineages producing retrovirus. Int J Cancer. 46(3):481-8. PMID: 2394515

Bibliography

Not specified

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: L35 (ECACC 91012319).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.