skip to main content

Karpas 620

Karpas 620

Catalogue No.

22110802

Cell Line Name

Karpas 620

Cell Line Description

Karpas 620 is a human plasma cell leukemia cell line. It was established from the peripheral blood of a 77-year-old woman at diagnosis of plasma cell leukemia (IgG kappa) in 1987.

Plasma cell leukemia is related to multiple myeloma.

The cultured cells have the ultra-structural appearance of plasma cells with abundant rough endoplasmic reticulum (RER) and secrete kappa light chain.

General Info

Species

Human

Unique to ECACC

No

Other Collection No.

Ximbio; 152425

Nagoya Status

Not Applicable

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Products

The line has the same hypotetraploid karyotype as the fresh cells from the patient. The cultured cells have the ultrastructural appearance of plasma cells with abundant rough endoplasmic reticulum (RER) and secrete kappa light chain. They are positive for surface antigens HLA DR, and WR17 (CD 37) and negative for CD1, CD3, CD4 and CD8.

Tissue of Origin

Peripheral blood

Morphology

Single round to polymorphic cells growing in suspension.

DNA profile (STR Profile)

Amelogenin: X,X
CSF1PO: 9.10
D3S1358: 14,16
D5S818: 9,11
D7S820: 9
D8S1179: 10,15
D13S317: 12,14
D16S539: 11,12
D18S51: 13,18
FGA: 26
Penta D: 10,13
Penta E: 14,15
TH01: 6,7
TPOX: 11,12
vWA: 17,19

Karyotype

47, +12, t(1;3;7)(p32.1;q21.1;q22.1), t(8;9)(q24.2;p13.3), t(14;18)(q32.3;q21.3)

Applications

Oncology research

Disease

Plasma cell leukemia

Culture Conditions

Subculture Routine

Maintain cultures between 5x10e4 and 9x10e5 cells per ml.

Doubling time approximately 60 hours.

Subculture every 2-4 days. 5% CO2, 37°C

Culture Medium

RPMI1640 + 10% Foetal Bovine serum (FBS) + 2mM L-Glutamine 

Growth Mode

Suspension

Additional Info

Depositor

Depositor: Dr Amanda Zeffman on behalf of Professor Abraham Karpas, Cambridge University. Originator: Professor Abraham Karpas University of Cambridge

Country of Origin

United Kingdom

GMO Status

Not Applicable

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

Bibliography

Stranks G., Height S.E., Mitchell P., Jadayel D.M., Yuille M.A.R., De Lord C., Clutterbuck R.D., Treleaven J.G., Powles R.L., Nacheva E., Oscier D.G., Karpas A., Lenoir G.M., Smith S.D., Millar J.L., Catovsky D., Dyer M.J.S. Deletions and rearrangement of CDKN2 in lymphoid malignancy. Blood 85:893-901(1995) PubMed=7849311

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Karpas 620 (ECACC 22110802).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.