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JIM1

JIM1

Catalogue No.

10032303

Cell Line Name

JIM1

Cell Line Description

JIM1 is a myeloma tumour line demonstrating typical plasma cell phenotype (CD38, PCA-1 8A and CD24 positive), deficient in DNA repair. JIM1 was derived from plasma myeloma cells derived from pleural fluid of advanced multiple myeloma patient, homoplastic with JIM3.

General Info

Species

Human

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Receptors

Not specified

Products

Not specified

Tissue of Origin

Blood

Morphology

Small spherical

Karyotype

Not specified

Applications

In vitro study and comparison of genetic instability in myeloma tumour lines (homoplastic with JIM3); in vitro study of effects of DNA repair deficiency in myeloma tumour lines

Disease

Myeloma

Culture Conditions

Subculture Routine

Seed at 2-9 x 10 5 cells/cm³ 5% CO₂; 37°C.

Culture Medium

Dexter culture medium, consisting of Fischer's medium + 20% FCS +10⁻⁷ M hydrocortisone sodium succinate.

Growth Mode

Suspension

Additional Info

Depositor

Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Hamilton MS, Barker HF, Ball J, Drew M, Abbot SD, Franklin IM. (1991) Normal and neoplastic human plasma cells express bcl-2 antigen. Leukemia. Sep; 5(9):768-71 PMID: 1943229.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: JIM1 (ECACC 10032303).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.