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Catalogue No.


Cell Line Name


Cell Line Description

Huh-7D12 is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D12 is owned by Imperial Innovations Ltd. This cell line cannot be commercially licensed.


Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11
D5S818: 12
D7S820: 11
D13S317: 10
D16S539: 10
TH01: 7
TPOX: 8,11
vWA: 16,18


That of original Huh-7


Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.


Hepatocellular Carcinoma

Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

Culture Medium

DMEM + 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Growth Mode


Additional Info


Prof John Monjardino, St Mary’s School of Medicine, c/o Imperial Innovations 52 Princes Gate, Exhibition Road, London SW7 2PG

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Cheng D, Yang A, Thomas H, Monjardino J.1993 Characterization of stable hepatitis delta expressing hepatoma cell lines: effect of HDAg on cell growth. Prog Clin Biol Res. 382:149-53. PMID: 8502673.


Cunha C, Monjardino J, Cheng D, Krause S, Carmo-Fonseca M. 1998 Localization of hepatitis delta virus RNA in the nucleus of human cells. RNA. 4(6):680-93. PMID: 9622127.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Huh-7D12 (ECACC 01042712).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.