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HL60 Ast.4

HL60 Ast.4

Catalogue No.

88120802

Cell Line Name

HL60 Ast.4

Cell Line Description

Variant of the promyelocytic cell line HL60 isolated after treating cells with alpha-particle radiation and then cloned in medium containing 1.25% DMSO. Treatment with 50nM TPA results in only very limited differentiation towards basophilic morphology (Giemsa stain). Differentiation towards monocyte lineage has not been observed.

Characteristics

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 13,14
D5S818: 12
D7S820: 11,12
D13S317: 8,11
D16S539: 11
TH01: 7,8
TPOX: 8,11
vWA: 16

Karyotype

2n = 48, hyperdiploid

Applications

Differentiation studies

Disease

Myelogenous Leukaemia

Culture Conditions

Cell Type

Promyeloid, Blast, Promyelocyte

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Do not allow the culture medium to become acidic.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

Growth Mode

Suspension

Additional Info

Depositor

Dr C M Bunce, Immunology Dept, University of Birmingham Medical School

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Exp Haematol 1983;11:828

Bibliography

Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HL60 Ast.4 (ECACC 88120802).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.