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Hep G2

Hep G2

Catalogue No.


Cell Line Name

Hep G2

Cell Line Description

The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. The cells secrete a variety of major plasma proteins e.g. albumin, alpha2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. They have been grown successfully in large scale cultivation systems. Hepatitis B virus surface antigens have not been detected. The cells will respond to stimulation with human growth hormone.

Access Cell line profile for this product.



Prothrombin, antithromin III, alpha-foetoprotein complement, C3 and C4 activator, fibrinogen

Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 10,11
D5S818: 11,12
D7S820: 10
D13S317: 9,13
D16S539: 12,13
TH01: 9
TPOX: 8,9
vWA: 17


Modal no. 55


Study of hepatocyte function and specific protein expression



Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Cells might take 6 days to form a confluent monolayer without media change if seeded at 1:4. Until then growth occurs mainly in islands.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Prof B Knowles, Wistar Institute, Philadelphia

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Nature 1979;282:615; Science 1980;209:497; In Vitro Cell Dev Biol 1987;23:349

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: Hep G2 (ECACC 85011430).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.