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Hep 3B

Hep 3B

Catalogue No.


Cell Line Name

Hep 3B

Cell Line Description

Derived from an 8 year old male. Cells contain integrated Hepatitis B virus genome. However there is currently no evidence that this cell line produces infectious Hepatitis B virus. The cells should be handled under laboratory containment level 2. Ethnicity: Black.



Alpha-foetoproteins, Hepatitis B Surface Antigen (HBSAg), albumin, transferrin, fibrinogen, complement (C3), alpha-2-macroglobulins

Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X
D5S818: 13
D7S820: 8,10
D13S317: 12,14
D16S539: 10
TH01: 6,7
vWA: 17


2n = 46, modal no. 60


Hepatocellular Carcinoma

Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Mr C B Morris, EMBL, Heidelburg

Country of Origin


Hazard Group (ACDP)

Hazard Group (ACDP) 2



Nature 1979;282:615; Science 1980;209:497


Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: Hep 3B (ECACC 86062703).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.