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HCT 116 BRCA2 -/- [42]

HCT 116 BRCA2 -/- [42]

Catalogue No.

16071901

Cell Line Name

HCT 116 BRCA2 -/- [42]

Cell Line Description

HCT 116 BRCA2 -/- [42] human colorectal carcinoma cells were generated by homologous recombination to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. These cells show phenotypes consistent with previous reports, including loss of Rad51 foci in the presence of double-strand breaks, chromosomal rearrangements and elevated sensitivity to the DNA-damaging agents phleomycin and Parp1 inhibitors. Clone 42 was generated at the same time as clone 46 cited in Xu et al., 2014. J Pathol. 2014 Nov;234(3):386-97. The originating laboratory have advised they observed no difference in culture phenotype between clone 42 and clone 46.

General Info

Species

Human

Unique to ECACC

Yes

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Products

Carcinoembryonic antigen (CEA1)

Tissue of Origin

Colon

Morphology

Polygonal epithelium

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 7,10
D3S1358: 12,13
D5S818: 10,11
D7S820: 11,12
D8S1179: 12,14
D13S317: 10,13
D16S539: 11,13
D18S51: 16,17
D21S11: 29,30
FGA : 18,23
Penta D: 9,13
Penta E: 13,14
TH01: 8,9
TPOX: 8
vWA: 17,21

Applications

HCT 116 BRCA2 -/- [42] human colorectal carcinoma cells were generated to study the role of BRCA2 in DNA repair and to identify additional functions of the BRCA2 gene. They will also be useful to identify potential therapeutic agents in cancer cells with BRCA2 mutations.

Disease

Carcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10⁴ cells/cm² using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO₂, and passaged every 3 to 4 days

Culture Medium

McCoy's 5a medium modified, with 10% foetal bovine serum and 2mM L-Glutamine

Growth Mode

Adherent

Additional Info

Depositor

Ximbio (formerly known as Cancer Research Technologies Limited)

Country of Origin

United Kingdom

GMO Status

Genetically Modified Organism Class 1 (GMO1)

Genetic Modification

HCT 116 BRCA2 -/- [42] human colorectal carcinoma cell line was generated by using two rounds of targeted disruption of the BRCA2 gene using homologous recombination with selectable marker cassettes

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Xu H, Xian J, Vire E, McKinney S, Wei V, Wong J, Tong R, Kouzarides T, Caldas C, Aparicio S.Up-regulation of the interferon-related genes in BRCA2 knockout epithelial cells. J Pathol. 2014 Nov;234(3):386-97. doi: 10.1002/path.4404. Epub 2014 Aug 28. DOI: 10.1002/path.4404. PMCID: PMC4882165. PMID: 25043256 [PubMed - indexed for MEDLINE]

Bibliography

Brattain MG, Fine WD, Khaled FM, Thompson J, Brattain DE. Heterogeneity of malignant cells from a human colonic carcinoma. Cancer Res. 1981 May; 41 (5):1751-6. PMID: 7214343 Brattain MG, et al. Enhancement of growth of human colon tumor cell lines by feeder layers of murine fibroblasts. J. Natl. Cancer Inst. 69: 767-771, 1982. PubMed: 6956756

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: HCT 116 BRCA2 -/- [42] (ECACC 16071901).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.