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HCT 116

HCT 116

Catalogue No.


Cell Line Name

HCT 116

Cell Line Description

One of 3 strains of malignant cells isolated from a male with colonic carcinoma. Cells are tumourigenic in nude mice and form colonies on agarose. Growth and plating efficiency are enhanced by using a feeder layer of murine fibroblasts (J.Natl. Cancer Inst.1982; 69:757-771)


Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 7,10
D5S818: 10,11
D7S820: 11,12
D13S317: 10,12
D16S539: 11,13
TH01: 8,9
vWA: 17,22


Modal no. 45


Tumourigenicity studies



Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

McCoy's 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Cancer Res 1981;41:1751; J Nat Cancer Inst 1982;69:767, Cancer 1995;76:201-209, Exp. Cell Res 1994; 214:215-224 , Cancer Res. 1997; 57:488-494 , Cancer Res. 1998; 58:95-101, Proc. Natl. Acad. Sci USA 93;4816-4820, Proc. Natl. Acad. Sci USA93: 8425-8430,


Cancer Res. 1997; 57:3562-3568. Abaan OD, Polley EC, Davis SR, Zhu YJ, Bilke S, Walker RL, Pineda M, Gindin Y, Jiang Y, Reinhold WC, Holbeck SL, Simon RM, Doroshow JH, Pommier Y, Meltzer PS.2013 The exomes of the NCI-60 panel: a genomic resource for cancer biology and systems pharmacology. Cancer Res. 73(14):4372-82. PMID: 23856246.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: HCT 116 (ECACC 91091005).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.