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Catalogue No.


Cell Line Name


Cell Line Description

H5 is a subclone of H4-II-E-C3 (ECACC catalogue no. 85061112) which originated from the Reuber H-35 hepatoma induced by chemical carcinogenesis in an A x C rat. The dedifferentiated H5 cells express high levels of glucose-6-phosphate dehydrogenase and exhibit a population doubling time of 12 hours. Differentiated variants are H4IIEC3 (85061112), Fao and others (see Biochimie 1974;56:1603 for details).


Tissue of Origin





Modal no. 52 (50-55)


Toxicity studies; negative control for differentiated hepatoma


Hepatocellular Carcinoma

Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:50 to 1:100 i.e. seeding at 1-2x1,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Culture Medium

Coon's modified Ham's F12 (or Kaign's modified Ham's F12 + 45mg/L ascorbic acid + 18mg/L Inositol) + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Dr M Weiss, Dept. Biologie Moleculaire, Institute Pasteur, Paris

Country of Origin


Hazard Group (ACDP)

Hazard Group (ACDP) 2



Biochimie 1974;56:1603; Cell Growth and Differentiation 1994;5:957

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: H5 (ECACC 94101905).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.