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EoL-1 cell

EoL-1 cell

Catalogue No.

94042252

Cell Line Name

EoL-1 cell

Cell Line Description

Differentiated by treatment with N-butyrate.

Characteristics

Tissue of Origin

Blood

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 7,10
D5S818: 11
D7S820: 10,13
D13S317: 12,13
D16S539: 8,10
TH01: 9.3
TPOX: 8
vWA: 16,17

Karyotype

Not specified

Disease

Eosinophilic Leukaemia

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Eol-1 cells grow very slowly and may take up to 5 days to reach a suitable level to subculture. It is best that these cells are left to grow and not agitated more than necessary.The cells actually grow as large clusters. They are beginning to reach confluence when the cells in the centre of each cluster start to look unhealthy. The change in media colour should also be an indication. Eol-1 cells prefer slightly acidic conditions and so may resuscitate better from frozen if started up in 15% foetal bovine serum.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Dr T Ohno, Riken Gene Bank, Tsukuba

Country of Origin

Japan

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Blood 1985;66:1233

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: EoL-1 cell (ECACC 94042252).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.