skip to main content



Catalogue No.


Cell Line Name


Cell Line Description

The cell line EB3 has been established in 1964 from a 3 year old male with Burkitt's lymphoma. Herpes-like virus particles have been detected by electron microscopy. Antibodies from patients with infectious mononucleosis react with the herpes type virus (EBV) found in EB3 cells. The cell line has been shown to be resistant to challenge with vesicular stomatitis virus. Lymphoblastoid, grow as single cells and in clumps. Ethnicity: Black.


Tissue of Origin



Single cells and clumps

DNA profile (STR Profile)

Amelogenin: X,Y
CSF1PO: 12,15
D5S818: 9,10
D7S820: 11
D13S317: 12,14
D16S539: 10,12
TH01: 7
TPOX: 6,9
vWA: 17,19


2n = 46


Study of Burkitt's lymphoma, virus sensitivity


Burkitt Lymphoma

Culture Conditions

Cell Type


Subculture Routine

Maintain cultures between 3-9 x 100,000 cells / ml; 5% CO₂; 37°C

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode


Additional Info


Obtained from ATCC

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Wistar Institute Symp. Monogr. 1965;4:59, PNAS USA 1968;59:94. Lancet 1964;1:252


Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: EB-3 (ECACC 90121003).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.