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UK Health Security Agency

CTLL-2

CTLL-2

Catalogue No.

93042610

Cell Line Name

CTLL-2

Cell Line Description

CTLL is a subclone of T cells derived from a C57bl/6 mouse. The cells require IL-2 for growth and are used to assay for its presence in conditioned media.

Characteristics

Tissue of Origin

Blood

Karyotype

Not specified

Disease

None Stated

Culture Conditions

Cell Type

Lymphoblastoid

Subculture Routine

Maintain cultures between 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Thaw ampoule and perform a cell count, spin out the DMSO and resuspend in an appropriate volume of media to give a cell concentration of 4-5 x 10⁵ cells/ml. Viability may be poor on resuscitation and may decrease further within the first 48 hours, the culture may appear non-viable at this time. Clusters of cells will eventually appear but this could take 1-2 weeks, during this time large media changes are not recommended. It may take 2-3 weeks for vigorous growth to be established. At subculture perform a cell count and maintain cultures between 3-9 x 10⁵ cells/ml, 5% CO₂, 37°C. For cryopreservation of CTLL-2, freeze at approximately 6 x 10⁶ cells/ml in 90% FBS + 10% DMSO.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% T-STIM or 10-20 IU/ml Human Recombinant IL-2 + 10% Foetal Bovine Serum (FBS).

Growth Mode

Suspension

Additional Info

Depositor

Prof D Kilburn, Department of Microbiology, University of Vancouver

Country of Origin

Canada

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Gillis S, Smith KA. 1977 Long term culture of tumour-specific cytotoxic T cells. Nature 268:154-6 PMID: 145543

Bibliography

Sladowski D, Steer SJ, Clothier RH, Balls M. 1993 An improved MTT assay. J Immunol Methods 157: 203-7 PMID: 8423364. Belani R, Weiner GJ. 1996 Expression of both B7-1 and CD28 contributes to the IL-2 responsiveness of CTLL-2 cells. Immunology 87: 271-4 PMID: 8698390

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CTLL-2 (ECACC 93042610).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.