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UK Health Security Agency

CMT 64

CMT 64

Catalogue No.

10032301

Cell Line Name

CMT 64

Cell Line Description

CMT 64 was isolated from a primary alveogenic lung carcinoma tumour mass in C57BL/lcrf mouse, achieving stable morphology and growth rate in culture, similar to the growth rate and morphology in the primary tumour, and in lung metastasis induced after subcutaneous inoculation of mice. CMT 64 cells can be maintained in completely defined serum-free medium. Tumours derived from tissue culture cells grown in serum-free and serum-containing medium can form lung metastases in appropriate animal host.

General Info

Species

Mouse

Release Conditions

Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

Characteristics

Tissue of Origin

Lung

Morphology

Closely packed sheets at confluence

Karyotype

Modal chromosome number 85

Applications

In vivo mouse tumourigenesis system for study of growth characteristics and metastasis of mouse tumour lines (homoplastic with stable CMT 167 line); stable growth rate and morphology in culture and in lung metastasis.

Disease

Glioblastoma/Astrocytoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at approximately 3 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density 1.2 x 10⁵ cells/cm²

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Licensed from: Cancer Research Technology Ltd Angel Building 407 St John Street London EC1V 4AD

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Franks LM, Carbonell AW, Hemmings VJ, Riddle PN. (1976) Metastasizing tumors from serum-supplemented and serum-free cell lines from a C57BL mouse lung tumor. Cancer Res. Mar; 36(3):1049-55. PMID: 1253168.

Bibliography

Zhang K, Wrzesinski K, Fey SJ, Mose Larsen P, Zhang X, Roepstorff P. (2008) Assessing CMT cell line stability by two dimensional polyacrylamide gel electrophoresis and mass spectrometry based proteome analysis. J Proteomics. Jul 21; 71(2):160-7 PMID: 18617143. Patel AC, Anna CH, Foley JF, Stockton PS, Tyson FL, Barrett JC, Devereux TR. (2000) Hypermethylation of the p16 (Ink4a) promoter in B6C3F1 mouse primary lung adenocarcinomas and mouse lung cell lines. Carcinogenesis. Sep; 21(9):1691-700 PMID: 1096410. Maschek U, Pülm W, Hämmerling GJ. (1989) Altered regulation of MHC class I genes indifferent tumor cell lines is reflected by distinct sets of DNase I hypersensitive sites. EMBO J. Aug;8(8):2297-304. PMID: 2507317 . Smith GJ, Lykke AW. (1985) Characterization of a neoplastic epithelial cell strain derived by dexamethasone treatment of cultured normal mouse type 2 pneumocytes. J. Pathol. Nov; 147(3):165-72 PMID: 4067735. Franks LM, Layton MG. (1984) Ultrastructural tumour differentiation and organ specificity in high and low metastatic lines from a mouse lung carcinoma. Br J Cancer. Apr; 49(4):423-9 PMID: 6324837. Layton MG, Franks LM. (1984) Heterogeneity in a spontaneous mouse lung carcinoma: selection and characterisation of stable metastatic variants. Br J Cancer. Apr; 49(4):415-21 PMID: 6324836. Steele JG, Rowlatt C, Sandall JK, Franks LM. (1983) Cell surface properties of high- and low-metastatic cell lines selected from a spontaneous mouse lung carcinoma. Int J Cancer. Dec 15; 32(6):769-79 PMID: 6654529.

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CMT 64 (ECACC 10032301).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.