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CD1B4

CD1B4

Catalogue No.

19072502

Cell Line Name

CD1B4

Cell Line Description

CD1B4 (also described in the literature as CDIB4) antibodies were isolated from centrioles and a small amount of pericentriolar material from lamb thymus cells. Monoclonal antibodies were raised by injecting denatured centriole preparations and screened using an immunofluorescence assay. The protein detected by the CD1B4 antibody is a single 96-KDa protein termed Cenexin. This is located at the base of the primary cilium, defining its association with the only mature centriole of the centriole pair. The antibody stains the mature centriole even when it is not forming a cilium and also is detected in cells which do not possess primary cilia. Immunogen: Denatured centrioles isolated from lamb thymus. Product-Antibody: Anti-cenexin. Antibody subclass: IgM.

Characteristics

Myeloma

Sp2-0/Ag-14

Immunogen

Denatured centrioles isolated from lamb thymus

Immunological Donor

Mouse spleen

Antibody Isotype

IgM

Morphology

Spherical

Tissue of Origin

Hybrid

Culture Conditions

Cell Type

Hybridoma

Subculture Routine

Maintain cultures between 1-9 x 100,000 cells/ml; 5% CO₂; 37°C

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Growth Mode

Suspension

Additional Info

Depositor

Keith Gull, Sir William Dunn School of Pathology,South Parks Road, Oxford OX1 3RE

Country of Origin

United Kingdom

Hazard Group (ACDP)

2

References

References

Lange, B.M.H and Gull, K. (1995): A molecular marker for centriole maturation in the mammalian cell cycle. Journal of Cell Biology, 130: 919-927.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: CD1B4 (ECACC 19072502)

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

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