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UK Health Security Agency

CACO-2

CACO-2

Catalogue No.

86010202

Cell Line Name

CACO-2

Cell Line Description

Isolated from a primary colonic tumour in a 72-year-old Caucasian male using the explant culture technique. Forms moderately well differentiated adenocarcinomas consistent with colonic primary grade II, in nude mice.NaviCyte Scientific holds the exclusive commercial distribution rights to the CACO-2 cell line deposited by the Memorial Sloan-Kettering Cancer Center. Note: All uses of Catalogue Numbers 86010202 and 09042001, other than for research by a non-commercial or academic entity, require a license and use authorization from NaviCyte Scientific under its exclusive arrangement with Memorial Sloan-Kettering Cancer Center. For information on the licensing terms, please contact NaviCyte Scientific via contact@navicyte-scientific.com or phone (+1) 973-868-6100.

General Info

Species

Human

Release Conditions

Restricted – all organisations other than for research by a non-commercial or academic entity, require a license and use authorization from NaviCyte Scientific under its exclusive arrangement with Memorial Sloan-Kettering Cancer Center. For information on the licensing terms, please contact NaviCyte Scientific via contact@navicyte-scientific.com or (+1) 973-868-6100

Characteristics

Tissue of Origin

Colon

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11
D5S818: 12,13
D7S820: 11,12
D13S317: 11,13,14
D16S539: 12,13
TH01: 6
TPOX: 9,11
vWA: 16,18

Karyotype

Hypertetraploid, modal no. 96

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

CACO-2 cells should be seeded at 2-4e+04 cells/cm2 on resuscitation. For maintenance of the log phase growth, cells should be seeded at 5e+03 cells/cm2 to 3e+04 cells/cm2. Cells should be subcultured using 0.05% trypsin or trypsin/EDTA when approximately 50% confluent (Natoli et al., 2012) and should not be allowed to exceed 80% confluence in order to avoid spontaneous differentiation. Cells may show the appearance of circular vacuoles in the cytoplasm. These can increase in frequency as the culture density increases to confluence. To reduce their frequency, media change confluent cultures after 2-3 days if not sub cultured. 

 

Recommended Culture Conditions: 5% CO2; 37oC.

 

NB: On resuscitation and post subculture, CACO-2 cells can take up to 72 hours before they adhere to the plasticware and begin to proliferate. 

 

Ref: Natoli, M. et al. Good Caco-2 cell culture practices. Toxicology in Vitro, 26(8), 1243–1246, 2012.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Dr J Clarke, AVRI, Pirbright

Country of Origin

United Kingdom

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871

Natoli, M. et al. Good Caco-2 cell culture practices. Toxicology in Vitro, 26(8), 1243–1246, 2012.

Bibliography

Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080 Adachi A, et al. Productive, persistent infection of human colorectal cell lines with human immunodeficiency virus. J. Virol. 61: 209-213, 1987. PubMed: 3640832 Trainer DL, et al. Biological characterization and oncogene expression in human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: CACO-2 (ECACC 86010202).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.