BRIN-BG5
BRIN-BG5
Cell Line Name
Cell Line Description
BRIN-BG5 is a hybrid cell line formed by the electrofusion of a primary culture of NEDH rat pancreatic islets with RINm5F (a cell line derived from a NEDH rat insuliinoma). BRIN-BG5 has been shown to be tumourigenic when transplanted into a SCID mouse host. BRIN-BG5 has been mycoplasma eradicated at ECACC and the stocks available for supply have undergone a further 10 passages without detection of mycoplasma.
General Info
Species
Rat
Links
Cellosaurus: CVCL_W093
Release Conditions
Characteristics
Receptors
Products
Tissue of Origin
Karyotype
Disease
Culture Conditions
Cell Type
Subculture Routine
Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-4 x 10⁴ cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Population doubling approx 28hrs. At confluence 10⁵ cells/cm² can be expected.
Culture Medium
RPMI-1640 + 2mM Glutamine + 10% FCS
Growth Mode
Additional Info
Depositor
Country of Origin
Hazard Group (ACDP)
Applications
References
McClenaghan NH, Barnett CR, O'Harte FP, Swanston-Flatt SK, Ah-Sing E, Flatt PR. 1996 Characteristics of BRIN-BG5 and BRIN-BG7, two novel glucose-responsive insulin-secreting cell lines produced by electrofusion. J Endocrinol.;148(3):409-17 PMID: 8778219
Bibliography
McClenaghan NH, Flatt PR 1999 Engineering cultured insulin-secreting pancreatic B-cell lines. J Mol Med. 77(1):235-43 PMID: 9930971.
Documents
Images
Available Formats
- Frozen
- Growing
- DNA-5µg (100ng/µl)
- DNA Panel
- RNA-5µg (100ng/µl)
Citation Guidance
If use of this culture results in a scientific publication, it should be cited in the publication as: BRIN-BG5 (ECACC 10033001).
Biosafety Information
Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.
Further Information
The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.
Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.