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Bacillus subtilis subsp. spizizenii

Bacillus subtilis subsp. spizizenii

Catalogue No.

NCTC 10400

Current Name

Bacillus subtilis subsp. spizizenii

Type Strain

Reference Strain

Original Strain Reference

PCI 219

General Info


(Ehrenberg 1835) Cohn 1872 (AL)

Other Collection Numbers

PCI 219, ATCC 6633, BUCSAV 425, LOC:UNITED, CCM 1999, DSM 347, HANKEY B14, IAM 1069, NCIB 8054, NR SMITH 231, WDCM 00003



Hazard Group (ACDP)

Hazard Group (ACDP) 2

Culture Conditions

Growth Conditions on Solid Media

Nutrient agar, 24 hours, 37°C, O₂

Biological and Genomic Info

Gram Stain Result



Gluconate: -

Enzyme Tests

Beta-galactosidase: +

Catalase: +

Lecithinase / alpha: -

Strain Data

(N. R. Smith 231, Hankey B 14, BUCSAV 425, ATCC 6633, NCIB 8054) NCIB in 1965 / ATCC / Assay of paromomycin sulphate, chlortetracycline, streptomycin viomycin and penicillin in milk / Produces subtilin / British Pharmacopoeia 1973, p. A104

Colony Appearance


Additional Info

Accession Date






Previous Catalogue Name

Bacillus subtilis



production of subtilin



Extended Bibliography

Sipos, R. Szekely, A.J. Palatinszky, M. Revesz, S. Marialigeti, K. Nikolausz, M., (2007), Effect of primer mismatch, annealing temperature and PCR cycle number on 16S rRNA gene-targetting bacterial community analysis, FEMS Microbiol Ecol, 341-50, 2007 Mamane-Gravetz, H. Linden, K.G., (2005), Relationship between physiochemical properties, aggregation and u.v. inactivation of isolated indigenous spores in water, J Appl Microbiol, 351-63, 2005 Dubnau, D. Smith, I. Morrel, P. Marmur, J., (1965), Gene conservation in Bacillus species. I. Conserved genetic and nucleic base sequence homologies., Proc. Natl. Acad. Sci. U.S.A., 491-498 (1978), European Pharmacopeia. Test for sterility. Revised Text. July Roberts, R.J., (1985), Restriction and modification enzymes and their recognition sequences., Nucl. Acids Res., r165-r200 FachnormenausschußMaterialprüfung(FNM)desDeutschenInstitutsfürNormungDIN, (1978), DIN 54 380. Prüfung auf Zusätze von antimikrobiellen Bestandteilen. Prüfung von Papier, Karton und Pappe. DeutschesInstitutfürNormungDIN.NormenausschußMedizin(NAMed), (1997), DIN 58959-7. Qualitätsmanagement in der medizinischen Mikrobiologie. Teil 7: Allgemeine Anforderungen an das Mitführen von Kontrollstämmen. Beiblatt 2: ATCC- und DSM-Nummern häufig verwendeter Kontrollstämme. Heinzmann, S. Entian, K.-D., (1998), Nisin immunity in Bacillus subtilis. Poster No. PA016 at VAAM-Meeting, Frankfurt. Stein, T.H. Entian, K.-D., (1998), Characterization of peptide antibiotics produced by Bacillus subtilis ATCC 6633. Poster No. PA019 at VAAM-Meeting, Frankfurt. (1973), Handbook of Microbiology Vol.III. Microbial products. (1975), Europäisches Arzneibuch, Band II, amtliche Ausgabe. Tittiger, F. Kingscote, B. Meldrum, B. Prior, M., (1975), Survey of antibiotic residues in Canadian slaughter animals., Can. J. Comp. Med., 178-182 Stein, T. Düsterhus, S. Stroh, A. Entian, K.-D., (1999), Identification and characterization of the structural gene sbo for, and the chemical properties of, subtilosin A, a bacteriocine produced by Bacillus subtilis ATCC 6633. Poster No. K-D 10 at VAAM-Conference, Göttingen. Lovett, P.S. Young, F.E., (1969), Identification of Bacillus subtilis NRRL B-3275 as a strain of Bacillus pumilus., J. Bacteriol., 658-661

Available Formats

  • Ampoule (Bacteria)

If use of this culture results in a scientific publication, it should be cited in the publication as: Bacillus subtilis subsp. spizizenii (NCTC 10400)

It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

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