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UK Health Security Agency

AR42J-B13

AR42J-B13

Catalogue No.

20100101

Cell Line Name

AR42J-B13

Cell Line Description

The AR42J-B13 cell is a readily expandable rat pancreatic acinar-like cell that differentiates on simple plastic culture substrata into replicatively-senescent hepatocyte-like (B-13/H) cells in response to glucocorticoid exposure (dexamethasone). It was derived from a transplantable tumour of a rat exocrine pancreas.

B-13/H cells express a variety of liver-enriched and liver-specific genes, many at levels similar to hepatocytes in vivo.

Furthermore, the B-13/H phenotype is maintained for at least several weeks in-vitro, in contrast to normal hepatocytes which rapidly de-differentiate under the same simple - or even under more complex - culture conditions.

The B-13/H hepatocyte can be identified upon differentiation by the addition of the glucocorticoid Dexamethasone.

Acinar cells are clusters of cells that resemble a many-lobed bundle of cells, that resemble a cluster if grapes.

The pancreatic acinar cell is a highly specialized cell structure developed for synthesis, storage, and secretion of the many digestive enzymes produced in the pancreas. The acinar cell arises from the same pancreatic progenitor as duct and islet cells and is tightly polarized.

Characteristics

Receptors

This cell line express the glucocorticoid receptor.

Products

These cells differentiate on simple plastic culture substrata into replicatively-senescent hepatocyte-like (B-13/H) cells in response to glucocorticoid exposure. B-13/H cells express a variety of liver-enriched and liver-specific genes, many at levels similar to hepatocytes in vivo

Tissue of Origin

Pancreatic and Hepatic

Morphology

B-13 phenotype, small spherical with indistinct nucleus. B-13/H phenotype, large epithelial with prominent nucleus.

Karyotype

B-13 cells are male cells. Studies by the Depositor indicated that B-13 cells had 84 chromosomes and were near-tetraploid. A typical karyotype from B-13 cells illustrates that there were a number of structural abnormalities evident, resulting in the identity of several chromosomes being ambiguous.

Applications

Unlimited supply of hepatocyte-like cells for liver and toxicology studies.

Disease

Carcinoma

Culture Conditions

Cell Type

B-13 (acinar), B-13/H (hepatocyte).

Subculture Routine

Split sub-confluent cultures at 70-80% confluency using 0.05% trypsin/EDTA solution.
Cells can be seeding at 2-4 x 10e4 cells/cm2.


Incubate cultures in 10% CO2 at 37°C.


Cells may grow at 5% CO2, however the higher CO2 concentration is recommended by ECACC as it is DMEM media.

Culture Medium

DMEM + 10% Foetal Bovine Serum (FBS).

Treatment with 10 nM Dexamethasone (DEX) results in the transdifferentiation to hepatocyte-like cells which is maximal (75–85% of all cells) after 14 days.

Growth Mode

B-13 proliferative. B-13/H replicatively senescent.

Additional Info

Depositor

Matthew Wright, Newcastle University. Originator: Kojima lab: H. Mashima, H. Shibata, T. Mine and I. Kojima.

Country of Origin

United Kingdom

GMO Status

Not Applicable

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Mashima, H. et al. (1996) Formation of insulin-producing cells from pancreatic acinar AR42J cells by hepatocyte growth factor Endocrinology, 137, 3969–3976. PMID: 8756573.

Shen CN, Slack JM, Tosh D. (2000) Molecular basis of transdifferentiation of pancreas to liver. Nat Cell Biol. Dec;2(12):879-87. PMID: 11146651.

Wallace K., et al. (2011) Serine/threonine protein kinase SGK1 in glucocorticoid-dependent transdifferentiation of pancreatic acinar cells to hepatocytes. J Cell Sci. 2011 Feb 1;124(Pt 3):405-13. PMID: 21224398

Bibliography

Fairhall., E.A. et al. (2013) The B-13 hepatocyte progenitor cell resists pluripotency induction and differentiation to non-hepatocyte cells. Toxicol. Res. 2, 308–320 

Fairhall E.A., et al. (2013). Adult human exocrine pancreas differentiation to hepatocytes – potential source of a human hepatocyte progenitor for use in toxicology research. Toxicology Research 2, 80 – 87.

Probert P.M., et al. (2014). A reversible model for periportal fibrosis and a refined alternative to bile duct ligation. Toxicology Research 3(2), 98 - 109.

Richter M., et al. (2015) Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system. Toxicol Res (Camb). Nov 18;5(1). PMID: 30090344.

Fairhall E.A., et al. (2016) Pancreatic B-13 Cell Trans-Differentiation to Hepatocytes Is Dependent on Epigenetic-Regulated Changes in Gene Expression. PLoS One. Mar 8;11(3): PMID: 26954030.

Leitch AC., et al.(2017) B-13 progenitor-derived hepatocytes (B-13/H cells) model lipid dysregulation in response to drugs and chemicals. Toxicology. Jul 1;386:120-132.PMID: 28552552.

Philip M. E. Probert, Stephanie K. Meyer, Fouzeyyah Alsaeedi, Andrew A. Axon, Emma A. Fairhall, Karen Wallace, Michelle Charles, Fiona Oakley, Paul A. Jowsey, Peter G. Blain, Matthew C. Wright, An expandable donor-free supply of functional hepatocytes for toxicology, Toxicology Research, Volume 4, Issue 2, March 2015, Pages 203–222, https://doi.org/10.1039/c4tx00214h

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: AR42J-B13 (ECACC 20100101).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

 

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.