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Catalogue No.


Cell Line Name


Cell Line Description

Established from undifferentiated thyroid carcinomas of a 67 year old female patient. Pathologically the carcinoma tissue contained residual well differentiated components suggesting well differentiated to undifferentiated carcinoma progression. Tumour suppresser genes p53, Rb, APC and MCC were analysed and sequence analysis confirmed a C:G to T:A transition at the first base of p53 gene codon 273. Loss of heterozygosity of tumour suppresser genes was not observed.


Tissue of Origin

Thyroid gland

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 9,12
D5S818: 10,13
D7S820: 8,10
D13S317: 9
D16S539: 10,11
TH01: 6,7
vWA: 14,16


Not specified


As p53 mutations associate with undifferentiated carcinoma but not well differentiated carcinoma during multistep carcinogenesis of the thyroid, these cell lines should prove useful for research into the role of gene mutation in malignant transformation.



Culture Conditions

Cell Type


Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Doubling time is 43 hours. Saturation density at confluency is 5.7x10,000 cells/cm².

Culture Medium

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Growth Mode


Additional Info


Dr M Akiyama, Radiobiology Dept, Radiation Effects Research Foundation, 5-2 Hijiyama Park, Minami-ku, Hiroshima 732

Country of Origin

United States

Hazard Group (ACDP)

Hazard Group (ACDP) 2



Cancer Res 1992;52:1369; Int J Oncology 1994;4:583

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 8305C (ECACC 94090183).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.