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293 N3S

293 N3S

Catalogue No.


Cell Line Name

293 N3S

Cell Line Description

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.



Adenovirus 5 early region (E1) antigens

Tissue of Origin


DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11
D5S818: 9
D7S820: 11,12
D13S317: 12,14
D16S539: 9
TH01: 7,9.3
TPOX: 11
vWA: 16,19


Not specified


None Stated

Culture Conditions

Subculture Routine

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO₂; 37°C. Cell doubling time is 30 hours.

Culture Medium

Joklik's MEM + 2mM Glutamine + 10% Horse Serum.

Growth Mode


Additional Info


Dr F L Graham, Department of Biology, McCaster University, Ontario

Country of Origin


GMO Status

Genetically Modified Organism Class 1 (GMO1)

Hazard Group (ACDP)

Hazard Group (ACDP) 2



J Gen Virol 1987; 68;937


Not specified

Available Formats

  • Frozen
  • DNA-5µg (100ng/µl)

If use of this culture results in a scientific publication, it should be cited in the publication as: 293 N3S (ECACC 92052131).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.