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Serum free cell lines

An area of regulatory concern in the biopharmaceutical production industry is the use of animal derived products during production processes. One of the sources of this problem is the presence of animal derived components in the media (e.g. serum and growth factors) used to grow cell lines which produce the required product.

For years media manufacturers have produced media designed to support the growth of cell lines without the addition of serum and more recently in completely animal component-free/chemically defined media even to the extent of being peptide-free. The lengthy adaptation process before such a media can be incorporated in a production process along with the unreliability of the early serum-free media have often been a barrier to change. For this reason and due to customer demand ECACC has been working with media suppliers to provide a range of common cell lines which have already been adapted to growth in serum and protein free media.

In addition to the regulatory concerns mentioned above removal of serum from cell culture offers many other advantages such as:

  • reduced process variability

  • removal of ill defined raw materials

  • removal of serum sourcing issues

  • reduced risk of allergic response in end product

  • produces a more reproducible system for studies into differentiation, regulation and proliferation

  • aids downstream processing of product

The below lists cell lines currently available adapted to growth in serum/protein free medium:


General Cell Collection 22 items found, displaying 1 to 20
Catalogue Number Name Useful Information
00102307 CHO (PROTEIN FREE) Hamster, Ovary, None Stated
02050101 MDCK-Protein Free Canine, Kidney, None Stated
03061601 NS0-Serum-Free Mouse, Other, Myeloma
03092501 Sp2/0.Ag14-TurboDoma Mouse, Not Specified, None Stated
03092502 P3X63Ag8.653-TurboDoma Mouse, Other, Myeloma
03092503 Vero-Hektor Monkey, Kidney, None Stated
04092902 COS-1-InVitrus Monkey, Kidney, None Stated
05011001 Sf9 TiterHigh AC free Insect, Ovary, None Stated
05011002 CHO/dhFr-AC-free Hamster, Ovary, None Stated
05011003 293 GTP-AC-free Human, Kidney, None Stated
05030202 Sf21 TiterHigh AC free Insect, Ovary, None Stated
05030204 293-Hektor Human, Kidney, None Stated
05062301 BHK21 Clone13-2P(Hektor) Hamster, Kidney, None Stated
05062302 BHK21-InVitrus Hamster, Kidney, None Stated
05063001 COS-7-InVitrus Monkey, Kidney, None Stated
06020202 NS0-Turbodoma Mouse, Other, Myeloma
07042701 PNT2 (SERUM FREE) Human, Prostate gland, None Stated
07052901 PNT1A (SERUM FREE) Human, Prostate gland, None Stated
08060101 Sp2/0-Ag14 (serum-free) Mouse, Spleen, None Stated
10021102 Bob Human, Prostate gland, Adenocarcinoma
Hybridoma Collection 1 items found, displaying 1 to 1
Catalogue Number Name Useful Information
92120704 HyGPD-YK-1-1 Anti-muscle glyceraldehyde-3-phosphate dehydrogenase, rabbit, IgG2b