The Medical Research Council (MRC)
The Medical Research Council (MRC) has a mission to improve human health through world-class medical research. MRC supports research across the biomedical spectrum, from fundamental lab-based science to clinical trials, and in all major disease areas. MRC work closely with the NHS and the UK health departments to deliver their mission and give a high priority to research that is likely to make a real difference to clinical practice and the health of the population.
MRC is part of UK Research and Innovation (UKRI). UKRI invests in research and innovation to enrich lives, drive economic growth, and create jobs and high-quality public services across the UK.
Hybridoma Clones and Monoclonal Antibodies
Within our reagents catalogue there are a wide range of hybridoma clones which can produce a variety of monoclonal antibodies. These can be used for non-commercial research and purchased directly from ECACC or licensed for commercial use. If you are interested in our range of hybridoma clones and monoclonal antibodies you can search through our extensive catalogue using either the name, clone, specificity, reactivity, isotype or host and you will find information on the application and publications for each antibody available within the ECACC repository.
Other cell lines
Some of our most popular cell lines are the different versions of the mouse myeloma, NS0. These can be used in research and commercial production of therapeutic biologics and other proteins, including as a fusion partner for generation of hybridomas to produce antibodies. It has successfully been used to produce approved biologic therapeutics. An example includes the CD4 targeting monoclonal antibody Trogarzo, which is used in the treatment of HIV-1 infection. There are two versions of NS0 available on the ECACC repository which are NS0 and NS0-Turbodoma, which is optimised to grow in a turbodoma medium.
Purchasing Options for Hybridoma Clones
Included are some of MRC’s most successful reagents.
1. Anti-human CD200 monoclonal antibody
ECACC Number: 03062505
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Clone: OX104 | Reactivity: Human | Isotype: IgG1 Host: Mouse
Description: Recognises CD200 cell surface antigen expressed by subset of lymphocytes, some endothelial cells and neurons
2. Anti-HLA A, B, C monoclonal Antibody
ECACC Number: 84112003
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Clone: W6/32 HL | Reactivity: Human; Rhesus macaque; Bovine; Feline; Cynomolgus monkey; Baboon | Isotype: IgG2a | Host: Mouse
Description: Directed against human leucocytes and recognises an antigenic determinant on the 43kDa polypeptide chains of HLA-A, B and C
3. OX86 Cell line
ECACC Number: 96110601
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Species: Mouse | Cell type: Hybridoma | Uses: OX-40 detection assay | Growth medium: RPMI 1640 + 2mM glutamine + 10-20% Foetal Bovine Serum (FBS)
Description: OX-86 was raised by immunising AO rats with recombinant mouse OX-40 antigen and subsequent fusion of spleen cells with NS1 mouse myeloma cells. OX-86 mAb can be used to examine the distribution of OX-40,CD134, a member of the tumour necrosis factor receptor/nerve growth factor receptor super family.
Purchasing Options for NS0 Cell lines
1. NS0 Cell line
ECACC Number: 85110503
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Species: Mouse | Cell type: Myeloma | Uses: Fusion partner for hybridomas; protein expression | Growth medium: RPMI + FBS
Description: NS0 is a cell line derived from murine myeloma. It is a non-Ig secreting, non-light chain-synthesising subclone of NS-1(P3/NS1/1-Ag4-1).
2. NS0 Turbodoma
ECACC Number: 06020202
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Species: Mouse | Cell type: Myeloma | Growth medium: Turbodoma medium
Decription: NS0 cell line is adapted to grow in the chemically defined protein- and peptide-free medium Turbodoma (Cell Culture Technologies). Cholesterol is not required as a supplement for this culture. This cell line is suitable for use as a fusion parent for serum-free generation of hybridomas.The NS0 cell line is the property of MRC. ECACC is unable to supply this cell line or derivatives of this cell line to commercial entities unless a license has been first secured from MRC, negotiated by LifeArc (formerly known as MRC Technology); requests for commercial use of this cell line will be forwarded to LifeArc.
Medical Research Council (MRC) Collection
Explore cell lines from Medical Research Council (MRC) here.