Cell culture reagents
The following list is provided to aid acquisition of reagents suitable for the culture of ECACC cell lines.
| Catalogue no. | Brand | Product | Description |
| A1720 | Sigma | G418 | G 418 disulfate salt |
| A5006 | Sigma | L-Arginine | L-Arginine |
| B1522 | Sigma | BME | Basal Medium Eagle |
| B9285 | Sigma | Budr | Bromo-deoxy Uridine |
| C2639 | Sigma | Freeze Media SF | Cell Freezing Medium – Serum free |
| C3867 | Sigma | Collagen Type I | Collagen Type I solution from rat tail |
| C5467 | Sigma | CHO PF Media | EX-CELL® ACF CHO Medium - Animal Component Free |
| C5533 | Sigma | Collagen Type IV | Collagen Type IV (from human placenta) |
| C8919 | Sigma | Collagen Type I | Collagen Type I (from calf skin) |
| D2650 | Sigma | DMSO | Dimethyl Sulphoxide (Hybri-Max™) |
| D5796 | Sigma | DMEM | DMEM, Dulbecco’s Modified Eagle’s Medium |
| D8062 | Sigma | DMEM:F12 (1:1) | DMEM:Nutrient Mix F12 Ham (1:1) ratio mix |
| D8537 | Sigma | PBS | Phosphate Buffered Saline |
| F2442 | Sigma | FBS / FCS | Foetal Bovine Serum / Foetal Calf Serum (USA Origin) |
| F4135 | Sigma | FBS HI | Foetal Bovine Serum – Heat Inactivated (USA Origin) |
| F4759 | Sigma | Fibronectin | Fibronectin |
| 21475025 | Gibco | Fischer’s Medium | Fischer’s Medium |
| F0855 | Biochrom | Coon’s F12 | Nutrient Mixture F-12 Ham Coon’s Modification |
| G1890 | Sigma | Gelatin | Gelatin from Porcine skin |
| G2025 | Sigma | Glycerol | Glycerol |
| G5154 | Sigma | GMEM | Glasgow Minimum Essential Medium |
| G7513 | Sigma | Glutamine | L-Glutamine |
| G9391 | Sigma | Gelatin | Gelatin from Bovine skin |
| H0137 | Sigma | HT | HT media supplement (50x) Hybri-Max™ |
| H0262 | Sigma | HAT | HAT media supplement (50x) Hybri-Max™ |
| H0887 | Sigma | HEPES | HEPES solution |
| H1270 | Sigma | HS | Horse Serum |
| H6909 | Sigma | Hydrocortisone | Hydrocortisone solution |
| H8142 | Sigma | HES | Hybridoma Enhancing Supplement |
| I0516 | Sigma | Insulin | Insulin solution from Bovine pancreas. |
| I3146 | Sigma | ITS | Insulin – Transferrin – Selenium |
| I3390 | Sigma | IDMEM | IMDM / Iscove’s Modified Dulbecco’s Medium |
| I7908 | Sigma | IL-2 | Interleukin-2 human |
| I9278 | Sigma | Insulin | Insulin Solution - Human |
| L5158 | Sigma | LIF | Leukemia Inhibitory Factor ( from mouse) |
| L5520 | Sigma | L15 | L-15 Medium (Leibovitz) |
| L9010 | Sigma | LEH | Lactalbumin Enzymatic Hydrolysate |
| M2154 | Sigma | M199 | Medium 199 |
| M4526 | Sigma | ALPHA MEM | Minimum Essential Medium Eagle’s – Alpha Modification |
| M4655 | Sigma | EMEM (EBSS) | Minimum Essential Medium Eagle’s (Earle’s Balanced Salt Solution) |
| M5775 | Sigma | EMEM (HBSS) | Minimum Essential Medium Eagle’s (Hank’s Balanced Salt Solution) |
| M6250 | Sigma | 2ME | 2 – Mercaptoethanol |
| M6395 | Sigma | MCDB 105 | MCDB 105 Medium |
| M6895 | Sigma | VITS (MEM) | MEM Vitamins |
| M7145 | Sigma | NEAA | Non Essential Amino Acids |
| M8028 | Sigma | JMEM | Minimum Essential Medium Eagle’s - Joklik Modification |
| M8167 | Sigma | SMEM | Minimum Essential Medium Eagle’s - Spinner Modification |
| M8403 | Sigma | McCoy’s 5A | McCoy’s 5A Medium Modified |
| N1140 | Sigma | NCTC 109 | NCTC 109 Medium |
| N3520 | Sigma | Kaighn’s F12 | Nutrient Mixture F-12 Ham Kaighn’s Modification |
| N4637 | Sigma | NBCS | New Born Calf Serum |
| N6013 | Sigma | Ham’s F10 | Nutrient Mixture F-10 Ham’s |
| N6658 | Sigma | Ham’s F12 | Nutrient Mixture F-12 Ham’s |
| P8139 | Sigma | PMA | Phorbol Myristate Acetate |
| R7256 | Sigma | VITS (RPMI) | RPMI Vitamins |
| R8758 | Sigma | RPMI 1640 | Roswell Park Memorial Institute |
| S8636 | Sigma | NaP | Sodium Pyruvate |
| S8761 | Sigma | NaHCO3 | Sodium Bicarbonate |
| S9895 | Sigma | SDM | Schneider’s Drosophila Medium |
| T3160 | Sigma | TC-100 | TC-100 Insect Medium |
| 25300 | Sigma | Trypsin/EDTA | 0.05% Trypsin-EDTA , phenol red |
| T6414 | Gibco | Trypsin Inhibitor | (Soybean) Trypsin Inhibitor |
| T8154 | Sigma | Trypan Blue | Trypan Blue |
| T8159 | Sigma | TPB | Tryptose Phosphate Broth |
| W1625 | Sigma | WMB 752/1 | Waymouth Medium MB 752/1 |
List updated: February 2019
Disclaimer: please note there are other suppliers of these reagents; ECACC is unable to endorse any specific manufacturer or supplier
Back to top
Copyright © Public Health England.