Culture Collections

General Cell Collection Detail

ECACC General Cell Collection: N_N20R1

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 17011212
Cell Line Name: N_N20R1
Organisation deposited by: Ximbio (formerly known as Cancer Research Technologies Limited)
Other Collection No.: No
Unique to ECACC: Yes
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: N_N20R1 (ECACC 17011212)
Keywords: MDM2 inhibitor, Nutlin-3, resistance, MDM2-application
Cell Line Description: N_N20R1 is a subpopulation of the NGP neuroblastoma line which is resistant to 20 µM Nutlin-3.The parental line NGP was derived from a metastatic site in the lung.
Species: Human
Tissue of Origin: Brain
Disease Status: Cancer, neuroblastoma
Donor Sex: Male
Donor Age: 2 1/2
CellType: Neuroblastoma
Morphology: Adherent cells growing in monolayers and clumps
Growth Mode: Adherent
DNA Profile: STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11, 12
D13S317: 8, 12
D16S539: 8, 11
D18S51: 15, 16
D21S11:32, 33.1
D3S1358: 17,17
D5S818: 11, 12
D7S820: 11, 11
D8S1179: 11, 12
FGA: 19, 23
Penta D: 8, 10
Penta E: 11, 15
THO1: 9, 9.3
TPOX: 6, 9
vWA: 16, 21
Genetic Modification: N/A
Hazard Group (ACDP): 2
Biosafety Information: Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance

Hyperlinks to Material Safety Data Sheets (MSDS) documents:
Frozen cell cultures MSDS
Growing cell cultures MSDS
Nucleic acids derived from cell cultures MSDS
Subculture Routine: Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x104cells/cm2using 0.05% trypsin/EDTA solution. Cells are cultured at 37°C, in 95% air and 5% CO2, and passaged every 3 to 4 days.

Freeze medium: Foetal Bovine Serum (FBS) 90% and DMSO 10%

Culture Medium: DMEM + 10% Heat Inactivated FBS
Depositor: Ximbio (formerly known as Cancer Research Technologies Limited)
Originator: Yes
Country: UK
References: Drummond CJ, Esfandiari A, Liu J, Lu X, Hutton C, Jackson J, Bennaceur K, Xu Q, Makimanejavali AR, Del Bello F, Piergentili A, Newell DR, Hardcastle IR, Griffin RJ, Lunec J.TP53 mutant MDM2-amplified cell lines selected for resistance to MDM2-p53 binding antagonists retain sensitivity to ionizing radiation. Oncotarget. 2016 Jul 19;7(29):46203-46218. doi: 10.18632/oncotarget PMID: 27323823
Cellosaurus Link: CVCL_HG08
Patents: None
Release Conditions: Restricted - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' release conditions form in the supporting documents section.

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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