skip to main content

HT29-MTX-E12

HT29-MTX-E12

Catalogue No.

12040401

Cell Line Name

HT29-MTX-E12

Cell Line Description

HT29 cells were differentiated into mature goblet cells using methotrexate. Mucous-secreting HT29-MTX subclones were isolated from this cell clone and characterized with regard to tight junction formation, development of confluent monolayers and production of a mucous layer. HT29-MTX-E12 provides a model system to study the influence of the mucous layer on nanoparticle diffusion.

Characteristics

Tissue of Origin

Colon

DNA profile (STR Profile)

Amelogenin: X
CSF1PO: 11,12
D5S818: 11,12
D7S820: 10
D13S317: 11
D16S539: 11,12
TH01: 6
TPOX: 8,9
vWA: 17

Disease

Adenocarcinoma

Culture Conditions

Cell Type

Epithelial

Subculture Routine

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.05% trypsin/EDTA; 7.5-10% CO₂; 37°C. Suggested seeding density 2-4x10,000 cells/cm².

Culture Medium

DMEM + 2mM Glutamine + 1% Non essential amino acids + 10% Foetal Bovine Serum (FBS).

Growth Mode

Adherent

Additional Info

Depositor

Professor David Brayden, Associate Professor of Drug Delivery and Kevin McMahon, Senior Technical Officer, University College Dublin, UCD School of Veterinary Medicine, Belfield, Dublin 4, IRELAND. Originator: Professor Per Artursson, Uppsala University, Sweden.

Country of Origin

Ireland

Hazard Group (ACDP)

Hazard Group (ACDP) 2

Applications

References

Behrens I, Stenberg P, Artursson P, Kissel T. 2001 Transport of lipophilic drug molecules in a new mucus-secreting cell culture model based on HT29-MTX cells. Pharm Res. 18:1138-45. PMID: 11587485.

Bibliography

Keely S, Rullay A, Wilson C, Carmichael A, Carrington S, Corfield A, Haddleton DM, Brayden DJ. 2005 In vitro and ex vivo intestinal tissue models to measure mucoadhesion of poly (methacrylate) and N-trimethylated chitosan polymers. Pharm Res. 22:38-49. PMID: 15771228.

Available Formats

  • Frozen

If use of this culture results in a scientific publication, it should be cited in the publication as: HT29-MTX-E12 (ECACC 12040401).

Unless specified otherwise, at ECACC we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines (Advisory Committee on Dangerous Pathogens) (UK). All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country. ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.