Culture Collections

General Cell Collection Detail

ECACC General Cell Collection: BICR 78

Supplied by: European Collection of Authenticated Cell Cultures (ECACC)
Culture Type: Cell line
Collection: ECACC General Collection
Catalogue No.: 04072111
Cell Line Name: BICR 78
Citation Guidance: If use of this culture results in a scientific publication, it should be cited in the publication as: BICR 78 (ECACC 04072111)
Keywords: Oral alveolus squamous carcinoma
Cell Line Description: Adherent keratinocyte cell line derived from a squamous cell carcinoma of the oral alveolus of a Caucasian male. Keratin and involucrin markers present. Known mutations: p53, p16 and p14ARF. Cells are not tumourigenic in athymic mice.
Species: Human
Tissue of Origin: Alveolus
CellType: Epithelial, keratinocyte
Growth Mode: Adherent
Karyotype: Aneuploid
Biosafety Information: Unless specified otherwise, at the European Collection of Authenticated Cell Cultures (ECACC) we routinely handle all of our cell lines at containment level 2 in accordance with the ACDP guidelines. ACDP = Advisory Committee on Dangerous Pathogens (UK)
All cell cultures have the potential to carry as yet unidentified adventitious agents. It is the responsibility of the end user to ensure that their facilities comply with biosafety regulations for their own country.

ACDP Guidance: Biological agents: Managing the risks in laboratories and healthcare premises.

Hyperlinks to MSDS documents:
Frozen cell cultures Material Safety Data Sheet
Growing cell cultures Material Safety Data Sheet
Nucleic acids derived from cell cultures Material Safety Data Sheet
Subculture Routine: Split sub-confluent cultures (70-80%) 1:10 - 1:50 using 0.05% trypsin/EDTA; 8% CO2; 37°C. Suggested seeding density 2 x 10,000 cells/cm2.

Culture cells on a feeder layer of lethally-irradiated or mitomycin C-treated 3T3 Swiss Albino cells (ECACC Catalogue number: 85022108). Feeder layers are prepared in the flasks at least 24 hours in advance of being required. Where frozen stocks of treated 3T3 Swiss Albino cells have been prepared, an ampoule is thawed in 37C water bath and the contents quickly transferred to a 15ml centrifuge tube. DMEM medium is added drop wise to 5ml. Cells are centrifuged at 150 x g for 5 minutes at Room Temperature. Cells are resuspended in 5ml of medium. Cells are counted and added to flasks containing the correct BICR growth medium at 1-3 x 104 cells/cm2.

Culture Medium: DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.4 micrograms/ml Hydrocortisone.
Depositor: Prof. Kenneth Parkinson, Cancer Research Campaign Beatson Laboratories, Garscube Estate, Switchback Road, Glasgow G61 1BD
Originator: Yes
Country: UK
References: Edington KG, Loughran OP, Berry IJ, Parkinson EK 1995 Cellular immortality: a late event in the progression of human squamous cell carcinoma of the head and neck associated with p53 alteration and a high frequency of allele loss. Mol Carcinog. 13(4):254-65 PMID: 7646764.
Additional Bibliography: Loughran O, Clark LJ, Bond J, Baker A, Berry IJ, Edington KG, Ly IS, Simmons R, Haw R, Black DM, Newbold RF, Parkinson EK 1997 Evidence for the inactivation of multiple replicative lifespan genes in immortal human squamous cell carcinoma keratinocytes Oncogene. 14(16):1955-64 PMID: 9150362. Munro J, Stott FJ, Vousden KH, Peters G, Parkinson EK 1999 Role of the alternative INK4A proteins in human keratinocyte senescence: evidence for the specific inactivation of p16INK4A upon immortalization.Cancer Res. 59(11):2516-21 PMID: 10363964
Patents: None specified by Depositor
Release Conditions: Yes - commercial organisations are required to complete the 'Cell Line Release Authorisation for Research Use in Commercial Organisations' form

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The Culture Collections represent deposits of cultures from world-wide sources. While every effort is made to ensure details distributed by Culture Collections are accurate, Culture Collections cannot be held responsible for any inaccuracies in the data supplied. References where quoted are mainly attributed to the establishment of the cell culture and not for any specific property of the cell line, therefore further references should be obtained regarding cell culture characteristics. Passage numbers where given act only as a guide and Culture Collections does not guarantee the passage number stated will be the passage number received by the customer.

Cultures supplied by Culture Collections are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

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