Culture Collections

Control strains for the microbiological testing of water samples for Pseudomonas aeruginosa

Pseudomonas aeruginosa tap imageFollowing several high profile cases in 2012, the Department of Health, in collaboration with the Health Protection Agency (now known as the UK Health Security Agency), issued advice to healthcare providers to assist in the control of potential Pseudomonas aeruginosa infection from taps and water systems. This is of particular significance in augmented care units (high dependency, adult, paediatric and neonatal critical care, renal, transplant, haemato-oncology and burns units).

The guidance now forms 'Part C' of Health Technical Memorandum 04-01: Safe water in healthcare (2016). It includes guidance on best practice for the microbiological examination of water samples for P. aeruginosa.


The use of reference materials as control strains is key in microbiological examinations:

· to validate methods and confirm that they are fit for their intended purpose

· to confirm that laboratories can properly operate methods before introducing them

· for quality control procedures for on-going monitoring of tests and calibrations


NCTC offer a range of control strains of Pseudomonas species cited by the World Data Centre for Micro-organisms (WDCM) as follows:


WDCM Ref NCTC Ref Species
WDCM 00024 NCTC 10332 Pseudomonas aeruginosa
WDCM 00025 NCTC 12934 Pseudomonas aeruginosa
WDCM 00026 NCTC 12924 Pseudomonas aeruginosa
WDCM 00114 NCTC 10662 Pseudomonas aeruginosa
WDCM 00115 NCTC 10038 Pseudomonas fluorescens
WDCM 00116 NCTC 10689 Pseudomonas fragi
WDCM 00117 NCTC 10936 Pseudomonas putida










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