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Bacteria Collection: Streptococcus sanguinis

NCTC Number: NCTC 7863
Current Name: Streptococcus sanguinis
Original Strain Reference: 7863
Other Collection No: ATCC 10556; DSM 20567
Previous Catalogue Name: Streptococcus sanguis
Type Strain: Yes
Family: Streptococcaceae
Hazard Group (ACDP): 2
Release Restrictions: Terms & Conditions of Supply of Microbial Pathogens: Safety
Antigenic Properties: serovar serotype 1
Conditions for growth on solid media: Columbia blood agar, 24-48 hours, 37°C, aerobic
Conditions for growth on liquid media: nutrient broth,37, facultative anaerobe
Isolated From: human, subacute bacterial endocarditis
Whole Genome Sequence: http://www.ebi.ac.uk/ena/data/view/ERS980035
Annotated Genome: ftp://ftp.sanger.ac.uk/pub/project/pathogens/NCTC3000/...
16S rRNA Gene Sequence: >gb|X53653|NCTC 7863|Streptococcus sanguis partial 16S ribosomal RNA.| ttaatgagagtttga... >gb|AF272245|ATCC 10556|Streptococcus sanguinis 16S-23S rRNA intergenic spacer, completesequence.| atcggaaggtgcggc... >gb|U02924|ATCC 10556|Streptococcus sanguis ATCC 10556 16S rRNA gene, partial sequence.| ccctattgttagttg... >gb|AF003928|ATCC 10556|Streptococcus sanguis 16S ribosomal RNA gene, complete sequence.| tgatcctggctcagg... >gb|AB002524|ATCC 10556|Streptococcus sanguis DNA for 16S rRNA, strain ATCC 10556.| cttgctcctcttgga... >gb|DQ303192|ATCC 10556|Streptococcus sanguinis strain ATCC 10556 16S ribosomal RNA gene,partial sequence.| gcttgctcttcttgg... >gb|AY347565|ATCC 10556|Streptococcus sanguinis strain ATCC 10556 16S-23S ribosomal RNAintergenic spacer, complete sequence.| ctaaggagtccgtaa... >gb|AB051017|ATCC 10556|Streptococcus sanguis DNA, 16S-23S rRNA intergenic spacer region.| ctaaggagtccgtaa...
23S rRNA Gene Sequence: >gb|AF272245|ATCC 10556|Streptococcus sanguinis 16S-23S rRNA intergenic spacer, completesequence.| atcggaaggtgcggc... >gb|AY347565|ATCC 10556|Streptococcus sanguinis strain ATCC 10556 16S-23S ribosomal RNAintergenic spacer, complete sequence.| ctaaggagtccgtaa... >gb|AB168126|ATCC 10556|Streptococcus sanguinis gene for 23S rRNA, complete sequence,strain:ATCC 10556.| ggttaagttagtaag... >gb|AB051017|ATCC 10556|Streptococcus sanguis DNA, 16S-23S rRNA intergenic spacer region.| ctaaggagtccgtaa...
Bibliography: WHITE J C & NIVEN C F JNR. 1946 J.BACT. 51 717; WASHBURN M R ET.AL. 1946
Extended Bibliography: showhide Show bibliography
Ref #: 19216
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic bacteria, including bacteria found in cerebrospinal fluid
Volume: 32
Page(s): 335-51
Year: 1994
Keyword(s): GENBANK/U02893 GENBANK/U02894 GENBANK/U02895 GENBANK/U02896 GENBANK/U02897 GENBANK/U02898 GENBANK/U02899 GENBANK/U02900 GENBANK/U02901 GENBANK/U02902 GENBANK/U02903 GENBANK/U02904 GENBANK/U02905 GENBANK/U02906 GENBANK/U02907 GENBANK/U02908 GENBANK/U02909 GENBANK/U02910 GENBANK/U02911 GENBANK/U02912 GENBANK/U02913 GENBANK/U02914 GENBANK/U02915 GENBANK/U02916 GENBANK/U02917 GENBANK/U02918 GENBANK/U02919 GENBANK/U02920 GENBANK/U02921 GENBANK/U02922 Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Humans Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were tested, along with three series of oligonucleotide probes to detect the PCR product. The first series of probes is broad in range and consists of a universal bacterial probe, a gram-positive probe, a Bacteroides-Flavobacterium probe, and two probes for other gram-negative species. The second series was designed to detect PCR products from seven major bacterial species or groups frequently causing meningitis: Neisseria meningitidis, Haemophilus influenzae, Streptococcus pneumoniae, S. agalactiae, Escherichia coli and other enteric bacteria, Listeria monocytogenes, and Staphylococcus aureus. The third series was designed for the detection of DNA from species or genera commonly considered potential contaminants of clinical samples, including cerebrospinal fluid (CSF): Bacillus, Corynebacterium, Propionibacterium, and coagulase-negative Staphylococcus spp. The primers amplified DNA from all 124 different species of bacteria tested. Southern hybridization testing of the broad-range probes with washes containing 3 M tetramethylammonium chloride indicated that this set of probes correctly identified all but two of the 102 bacterial species tested, the exceptions being Deinococcus radiopugnans and Gardnerella vaginalis. The gram-negative and gram-positive probes hybridized to isolates of two newly characterized bacteria, Alloiococcus otitis and Rochalimaea henselii, as predicted by Gram stain characteristics. The CSF pathogen and contaminant probe sequences were compared with available sequence information and with sequencing data for 32 different species. Testing of the CSF pathogen and contaminant probes against DNA from over 60 different strains indicated that, with the exception of the coagulase-negative Staphylococcus probes, these probes provided the correct identification of bacterial species known to be found in CSF.
URL: 7512093
Ref #: 95509
Author(s): Majewski,J.;Zawadzki,P.;Pickerill,P.;Cohan,F.M.;Dowson,C.G.
Journal: J Bacteriol
Title: Barriers to genetic exchange between bacterial species: Streptococcus pneumoniae transformation
Volume: 182
Page(s): 1016-23
Year: 2000
Keyword(s): GENBANK/AF194507 GENBANK/AF194508 GENBANK/AF194509 GENBANK/AF194510 GENBANK/AF194511 GENBANK/AF194512 GENBANK/AF194513 GENBANK/AF194514 GENBANK/AF194515 GENBANK/AF194516 GENBANK/AF194517 GENBANK/AF194518 GENBANK/AF194519 GENBANK/AF194520 GENBANK/AF194521 GENBANK/AF194522 GENBANK/AF194523 GENBANK/AF194524 GENBANK/AF194525 GENBANK/AF194526 GENBANK/AF194527 GENBANK/AF194528 Base Pair Mismatch/*genetics DNA Repair/*genetics DNA, Bacterial/genetics DNA-Directed RNA Polymerases/genetics Molecular Sequence Data Phylogeny Recombination, Genetic Sequence Analysis, DNA Streptococcus/genetics Streptococcus pneumoniae/*genetics/growth & development *Transformation, Bacterial
Remarks: Interspecies genetic exchange is an important evolutionary mechanism in bacteria. It allows rapid acquisition of novel functions by transmission of adaptive genes between related species. However, the frequency of homologous recombination between bacterial species decreases sharply with the extent of DNA sequence divergence between the donor and the recipient. In Bacillus and Escherichia, this sexual isolation has been shown to be an exponential function of sequence divergence. Here we demonstrate that sexual isolation in transformation between Streptococcus pneumoniae recipient strains and donor DNA from related strains and species follows the described exponential relationship. We show that the Hex mismatch repair system poses a significant barrier to recombination over the entire range of sequence divergence (0.6 to 27%) investigated. Although mismatch repair becomes partially saturated, it is responsible for 34% of the observed sexual isolation. This is greater than the role of mismatch repair in Bacillus but less than that in Escherichia. The remaining non-Hex-mediated barrier to recombination can be provided by a variety of mechanisms. We discuss the possible additional mechanisms of sexual isolation, in view of earlier findings from Bacillus, Escherichia, and Streptococcus.
URL: 10648528
Ref #: 95488
Author(s): Whiley,R.A.;Fraser,H.Y.;Douglas,C.W.;Hardie,J.M.;Williams,A.M.;Collins,M.D.
Journal: FEMS Microbiol Lett
Title: Streptococcus parasanguis sp. nov., an atypical viridans Streptococcus from human clinical specimens
Volume: 56
Page(s): 115-21
Year: 1990
Keyword(s): Base Sequence DNA, Bacterial/genetics Humans Molecular Sequence Data RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Homology, Nucleic Acid Serotyping Streptococcus/*classification/genetics
Remarks: Molecular taxonomic studies were performed on ten strains of an unusual 'viridans streptococcus' that were originally isolated from human throats, blood and urine. On the basis of DNA-DNA hybridization studies the strains formed a single homology group distinct from all recognized species of oral and viridans streptococci. 16S ribosomal RNA reverse transcriptase sequence studies confirmed the genealogical distinctiveness of the human strains. The results of the present study clearly demonstrate that the human strains represent a new species of the viridans group for which the name Streptococcus parasanguis sp. nov. is proposed. The type strain is ATCC 15912.
URL: 1692001
Ref #: 95478
Author(s): Pan,Y.P.;Li,Y.;Caufield,P.W.
Journal: Oral Microbiol Immunol
Title: Phenotypic and genotypic diversity of Streptococcus sanguis in infants
Volume: 16
Page(s): 235-42
Year: 2001
Keyword(s): Bacterial Typing Techniques Chromosome Mapping Chromosomes, Bacterial/genetics Cohort Studies Culture Media DNA Fingerprinting DNA, Bacterial/analysis DNA, Intergenic/genetics Genotype Humans Infant Infant, Newborn Longitudinal Studies Mouth/*microbiology Phenotype Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/genetics RNA, Ribosomal, 23S/genetics Sequence Analysis, DNA Statistics Streptococcus sanguis/classification/*genetics Variation (Genetics)
Remarks: Streptococcus sanguis comprises a heterogeneous group of oral streptococci indigenous to the oral cavity of humans. A total of 289 isolates from an infant population (n=37) were tentatively identified as S. sanguis on the basis of the distinctive colony morphology as shown on MM10-sucrose non-selective medium. These isolates were divided into four biovars of S. sanguis as determined by an extended panel of biochemical attributes. Chromosomal DNA was extracted from each isolate, and an AP-PCR fingerprint profile was obtained to allow study of the diversity within and among the infants. In this study, all four biovars of S. sanguis were detected in the infants. A wide genotypic diversity of S. sanguis was observed among these isolates; on average, each infant harbored 2.7 unique amplitypes as shown by the AP-PCR fingerprints. To explore the phylogenic relationship among these S. sanguis isolates, 20 strains representing the four biovars were selected at random for sequencing of their 16S rDNA and 16S-23S rDNA intergenic spacer chromosomal loci. Two major sequence patterns were identified within the 16S rDNA sequences. A phylogenic analysis showed that members from each of the four biovars of S. sanguis bore close relationship with the type-strain ATCC 10556 sequence, and that all of the isolates representing the four biovars could be clustered into two main phylotypes. The biovars were distributed throughout the phylotypes, indicating no correlation between the genetic and phenotypic groupings.
URL: 11442849
Ref #: 17178
Author(s): Kawata,K.;Anzai,T.;Senna,K.;Kikuchi,N.;Ezawa,A.;Takahashi,T.
Journal: FEMS Microbiol Lett
Title: Simple and rapid PCR method for identification of streptococcal species relevant to animal infections based on 23S rDNA sequence
Volume: 237
Page(s): 57-64
Year: 2004
Keyword(s): GENBANK/AB168118 GENBANK/AB168119 GENBANK/AB168120 GENBANK/AB168121 GENBANK/AB168122 GENBANK/AB168123 GENBANK/AB168124 GENBANK/AB168125 GENBANK/AB168126 GENBANK/AB168127 GENBANK/AB168128 Animals DNA, Bacterial/*analysis/chemistry DNA, Ribosomal/*analysis/chemistry Electrophoresis, Agar Gel Genes, rRNA Molecular Sequence Data Phylogeny Polymerase Chain Reaction/*methods RNA, Bacterial/genetics RNA, Ribosomal, 23S/genetics Sequence Analysis, DNA Streptococcal Infections/microbiology/*veterinary Streptococcus/*classification/genetics/*isolation & purification
Remarks: A PCR identification system targeting 23S rDNA sequences for the identification of eight streptococcal species relevant to animal infections (Streptococcus agalactiae, S. bovis, S. canis, S. dysgalactiae, S. equi, S. porcinus, S. suis and S. uberis) was developed. This system consists of two PCR reactions, A and B, in which seven and eight primers, respectively, are used simultaneously, and was designed so that each amplification product indicates a species by its size. A total of 111 cultures, including the type strain of eight species, could be successfully identified and differentiated as individual species, except for the cross reactivity between S. bovis and S. equinus. The developed PCR system can complete the identification procedure for eight streptococcal species through two tube reactions per isolate, and, therefore, might provide a rapid, simple and accurate diagnostic tool for veterinary laboratories.
URL: 15268938
Ref #: 82554
Author(s): Chen,C.C.;Teng,L.J.;Chang,T.C.
Journal: J Clin Microbiol
Title: Identification of clinically relevant viridans group streptococci by sequence analysis of the 16S-23S ribosomal DNA spacer region
Volume: 42
Page(s): 2651-7
Year: 2004
Keyword(s): DNA, Ribosomal Spacer/*chemistry Humans Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S/*genetics RNA, Ribosomal, 23S/*genetics Sequence Analysis, DNA Viridans Streptococci/classification/genetics/*isolation & purification
Remarks: The feasibility of sequence analysis of the 16S-23S ribosomal DNA (rDNA) intergenic spacer (ITS) for the identification of clinically relevant viridans group streptococci (VS) was evaluated. The ITS regions of 29 reference strains (11 species) of VS were amplified by PCR and sequenced. These 11 species were Streptococcus anginosus, S. constellatus, S. gordonii, S. intermedius, S. mitis, S. mutans, S. oralis, S. parasanguinis, S. salivarius, S. sanguinis, and S. uberis. The ITS lengths (246 to 391 bp) and sequences were highly conserved among strains within a species. The intraspecies similarity scores for the ITS sequences ranged from 0.98 to 1.0, except for the score for S. gordonii strains. The interspecies similarity scores for the ITS sequences varied from 0.31 to 0.93. Phylogenetic analysis of the ITS regions revealed that evolution of the regions of some species of VS is not parallel to that of the 16S rRNA genes. One hundred six clinical isolates of VS were identified by the Rapid ID 32 STREP system (bioMerieux Vitek, Marcy l'Etoile, France) and by ITS sequencing, and the level of disagreement between the two methods was 18% (19 isolates). Most isolates producing discrepant results could be unambiguously assigned to a specific species by their ITS sequences. The accuracy of using ITS sequencing for identification of VS was verified by 16S rDNA sequencing for all strains except strains of S. oralis and S. mitis, which were difficult to differentiate by their 16S rDNA sequences. In conclusion, identification of species of VS by ITS sequencing is reliable and could be used as an alternative accurate method for identification of VS.
URL: 15184447
Ref #: 82526
Author(s): Hoshino,T.;Fujiwara,T.;Kilian,M.
Journal: J Clin Microbiol
Title: Use of phylogenetic and phenotypic analyses to identify nonhemolytic streptococci isolated from bacteremic patients
Volume: 43
Page(s): 6073-85
Year: 2005
Keyword(s): GENBANK/AB199330 GENBANK/AB199331 GENBANK/AB199332 GENBANK/AB199333 GENBANK/AB199334 GENBANK/AB199335 GENBANK/AB199336 GENBANK/AB199337 GENBANK/AB199338 GENBANK/AB199339 GENBANK/AB199340 GENBANK/AB199341 GENBANK/AB199342 GENBANK/AB199343 GENBANK/AB199344 GENBANK/AB199345 GENBANK/AB199346 GENBANK/AB199347 GENBANK/AB199348 GENBANK/AB199349 GENBANK/AB199350 GENBANK/AB199351 GENBANK/AB199352 GENBANK/AB199353 GENBANK/AB199354 GENBANK/AB199355 GENBANK/AB199356 GENBANK/AB199357 GENBANK/AB199358 GENBANK/AB199359 GENBANK/AB199360 GENBANK/AB199361 GENBANK/AB199362 GENBANK/AB199363 GENBANK/AB199364 GENBANK/AB199365 GENBANK/AB199366 GENBANK/AB199367 GENBANK/AB199368 GENBANK/AB199369 GENBANK/AB199370 GENBANK/AB199371 GENBANK/AB199372 GENBANK/AB199373 GENBANK/AB199374 GENBANK/AB199375 GENBANK/AB199376 GENBANK/AB199377 GENBANK/AB199378 GENBANK/AB199379 GENBANK/AB199380 GENBANK/AB199381 GENBANK/AB199382 GENBANK/AB199383 GENBANK/AB199384 GENBANK/AB199385 GENBANK/AB199386 GENBANK/AB199387 GENBANK/AB199388 GENBANK/AB199389 GENBANK/AB199390 GENBANK/AB199391 GENBANK/AB199392 GENBANK/AB199393 GENBANK/AB199394 GENBANK/AB199395 GENBANK/AB199396 GENBANK/AB199397 GENBANK/AB199398 GENBANK/AB199399 GENBANK/AB199400 GENBANK/AB199401 GENBANK/AB199402 GENBANK/AB199403 GENBANK/AB199404 GENBANK/AB199405 GENBANK/AB199406 GENBANK/AB199407 GENBANK/AB199408 GENBANK/AB199409 GENBANK/AB199410 GENBANK/AB199411 GENBANK/AB199412 GENBANK/AB199413 GENBANK/AB199414 GENBANK/AB199415 GENBANK/AB199416 GENBANK/AB199417 GENBANK/AB199418 GENBANK/AB199419 GENBANK/AB199420 GENBANK/AB199421 GENBANK/AB199422 GENBANK/AB199423 GENBANK/AB199424 GENBANK/AB199425 GENBANK/AB199426 GENBANK/AB199427 GENBANK/AB199428 GENBANK/AB199429 GENBANK/AB199430 GENBANK/AB199431 GENBANK/AB199432 GENBANK/AB199433 GENBANK/AB199434 GENBANK/AB199435 GENBANK/AB199436 GENBANK/AB199437 GENBANK/AB199438 GENBANK/AB199439 GENBANK/AB199440 GENBANK/AB199441 GENBANK/AB199442 GENBANK/AB199443 GENBANK/AB199444 GENBANK/AB199445 GENBANK/AB199446 GENBANK/AB199447 GENBANK/AB199448 GENBANK/AB199449 GENBANK/AB199450 GENBANK/AB199451 GENBANK/AB199452 GENBANK/AB199453 GENBANK/AB199454 GENBANK/AB199455 GENBANK/AB199456 GENBANK/AB199457 GENBANK/AB199458 GENBANK/AB199459 GENBANK/AB199460 GENBANK/AB199461 GENBANK/AB199462 GENBANK/AB199463 GENBANK/AB199464 GENBANK/AB199465 GENBANK/AB199466 GENBANK/AB199467 GENBANK/AB199468 GENBANK/AB199469 GENBANK/AB199470 GENBANK/AB199471 GENBANK/AB199472 GENBANK/AB199473 GENBANK/AB199474 GENBANK/AB199475 GENBANK/AB199476 GENBANK/AB199477 GENBANK/AB199478 GENBANK/AB199479 GENBANK/AB199480 GENBANK/AB199481 GENBANK/AB199482 GENBANK/AB199483 GENBANK/AB199484 GENBANK/AB199485 GENBANK/AB199486 GENBANK/AB199487 GENBANK/AB199488 GENBANK/AB199489 GENBANK/AB199490 GENBANK/AB199491 GENBANK/AB199492 GENBANK/AB199493 GENBANK/AB199494 GENBANK/AB199495 GENBANK/AB199496 GENBANK/AB199497 GENBANK/AB199498 GENBANK/AB199499 GENBANK/AB199500 GENBANK/AB199501 GENBANK/AB199502 GENBANK/AB199503 GENBANK/AB199504 GENBANK/AB199505 GENBANK/AB199506 GENBANK/AB199507 GENBANK/AB199508 GENBANK/AB199509 GENBANK/AB199510 GENBANK/AB199511 GENBANK/AB199512 GENBANK/AB199513 GENBANK/AB199514 GENBANK/AB199515 GENBANK/AB199516 GENBANK/AB199517 GENBANK/AB199518 GENBANK/AB199519 GENBANK/AB199520 GENBANK/AB199521 GENBANK/AB199522 GENBANK/AB199523 GENBANK/AB199524 GENBANK/AB199525 GENBANK/AB199526 GENBANK/AB199527 GENBANK/AB199528 GENBANK/AB199529 GENBANK/AB199530 GENBANK/AB199531 GENBANK/AB199532 GENBANK/AB199533 GENBANK/AB199534 GENBANK/AB199535 GENBANK/AB199536 GENBANK/AB199537 GENBANK/AB199538 GENBANK/AB199539 GENBANK/AB199540 GENBANK/AB199541 GENBANK/AB199542 GENBANK/AB199543 GENBANK/AB199544 GENBANK/AB199545 GENBANK/AB199546 GENBANK/AB199547 GENBANK/AB199548 GENBANK/AB199914 GENBANK/AB199915 GENBANK/AB199916 GENBANK/AB199917 GENBANK/AB199918 GENBANK/AB199919 GENBANK/AB199920 GENBANK/AB199921 GENBANK/AB199922 GENBANK/AB199923 GENBANK/AB199924 GENBANK/AB199925 GENBANK/AB199926 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GENBANK/AB200101 GENBANK/AB200102 GENBANK/AB200103 GENBANK/AB200104 GENBANK/AB200105 GENBANK/AB200106 GENBANK/AB200107 GENBANK/AB200108 GENBANK/AB200109 GENBANK/AB200110 GENBANK/AB200111 GENBANK/AB200112 GENBANK/AB200113 GENBANK/AB200114 GENBANK/AB200115 GENBANK/AB200116 GENBANK/AB200117 GENBANK/AB200118 GENBANK/AB200119 GENBANK/AB200120 GENBANK/AB200121 GENBANK/AB200122 GENBANK/AB200123 GENBANK/AB200124 GENBANK/AB200125 GENBANK/AB200126 GENBANK/AB200127 GENBANK/AB200128 GENBANK/AB200129 GENBANK/AB200130 GENBANK/AB200131 GENBANK/AB200132 GENBANK/AB200133 GENBANK/AB200134 GENBANK/AB200135 GENBANK/AB200136 GENBANK/AB200137 GENBANK/AB200138 GENBANK/AB200139 GENBANK/AB200140 GENBANK/AB200141 GENBANK/AB200142 GENBANK/AB200143 GENBANK/AB200144 GENBANK/AB200145 GENBANK/AB200146 GENBANK/AB200147 GENBANK/AB200148 GENBANK/AB200149 GENBANK/AB200150 GENBANK/AB200151 GENBANK/AB200152 GENBANK/AB200153 GENBANK/AB200154 GENBANK/AB200155 GENBANK/AB200156 GENBANK/AB200157 GENBANK/AB200158 GENBANK/AB200159 GENBANK/AB200160 GENBANK/AB200161 GENBANK/AB200162 GENBANK/AB200163 GENBANK/AB200164 GENBANK/AB200165 GENBANK/AB200166 GENBANK/AB200167 GENBANK/AB218984 GENBANK/AB218985 Bacteremia/*microbiology Bacterial Proteins/genetics *Bacterial Typing Techniques *Hemolysis Humans Molecular Sequence Data Phenotype *Phylogeny Polymerase Chain Reaction/methods Reagent Kits, Diagnostic Sequence Analysis, DNA Species Specificity Streptococcal Infections/microbiology Streptococcus/*classification/genetics/isolation & purification/*physiology
Remarks: The aim of this study was to evaluate molecular and phenotypic methods for the identification of nonhemolytic streptococci. A collection of 148 strains consisting of 115 clinical isolates from cases of infective endocarditis, septicemia, and meningitis and 33 reference strains, including type strains of all relevant Streptococcus species, were examined. Identification was performed by phylogenetic analysis of nucleotide sequences of four housekeeping genes, ddl, gdh, rpoB, and sodA; by PCR analysis of the glucosyltransferase (gtf) gene; and by conventional phenotypic characterization and identification using two commercial kits, Rapid ID 32 STREP and STREPTOGRAM and the associated databases. A phylogenetic tree based on concatenated sequences of the four housekeeping genes allowed unequivocal differentiation of recognized species and was used as the reference. Analysis of single gene sequences revealed deviation clustering in eight strains (5.4%) due to homologous recombination with other species. This was particularly evident in S. sanguinis and in members of the anginosus group of streptococci. The rate of correct identification of the strains by both commercial identification kits was below 50% but varied significantly between species. The most significant problems were observed with S. mitis and S. oralis and 11 Streptococcus species described since 1991. Our data indicate that identification based on multilocus sequence analysis is optimal. As a more practical alternative we recommend identification based on sodA sequences with reference to a comprehensive set of sequences that is available for downloading from our server. An analysis of the species distribution of 107 nonhemolytic streptococci from bacteremic patients showed a predominance of S. oralis and S. anginosus with various underlying infections.
URL: 16333101
Ref #: 84110
Author(s): Bekal,S.;Gaudreau,C.;Laurence,R.A.;Simoneau,E.;Raynal,L.
Journal: J Clin Microbiol
Title: Streptococcus pseudoporcinus sp. nov., a novel species isolated from the genitourinary tract of women
Volume: 44
Page(s): 2584-6
Year: 2006
Keyword(s): GENBANK/DQ303183 GENBANK/DQ303184 GENBANK/DQ303185 GENBANK/DQ303186 GENBANK/DQ303187 GENBANK/DQ303188 GENBANK/DQ303189 GENBANK/DQ303190 GENBANK/DQ303191 GENBANK/DQ303192 GENBANK/DQ303193 GENBANK/DQ303194 GENBANK/DQ303195 GENBANK/DQ303196 GENBANK/DQ303197 GENBANK/DQ303198 GENBANK/DQ303199 GENBANK/DQ303200 GENBANK/DQ303201 GENBANK/DQ303202 GENBANK/DQ303203 GENBANK/DQ303204 GENBANK/DQ303205 GENBANK/DQ303206 GENBANK/DQ303207 GENBANK/DQ303208 GENBANK/DQ303209 GENBANK/DQ340843 Cluster Analysis DNA, Bacterial/chemistry/genetics DNA, Ribosomal/chemistry/genetics Female Female Urogenital Diseases/*microbiology Genes, rRNA Humans Molecular Sequence Data Phylogeny RNA, Bacterial/genetics RNA, Ribosomal, 16S/genetics Sequence Analysis, DNA Streptococcal Infections/*microbiology Streptococcus/*classification/genetics/*isolation & purification Urogenital System/*microbiology
Remarks: Streptococcus strains from animal and human sources identified biochemically as Streptococcus porcinus were investigated by 16S rRNA gene sequencing. The nine human strains isolated between 1997 and 2005 formed a single cluster with more than 2.1% dissimilarity with S. porcinus strains from animal sources. A novel species, Streptococcus pseudoporcinus sp. nov., is proposed.
URL: 16825387
Ref #: 13687
Author(s): Greisen,K.;Loeffelholz,M.;Purohit,A.;Leong,D.
Journal: J Clin Microbiol
Title: PCR primers and probes for the 16S rRNA gene of most species of pathogenic
Volume: 32
Page(s): 335-351
Year: 1994
Keyword(s): 0 (DNA Primers) 0 (DNA Probes) 0 (RNA, Bacterial) 0 (RNA, Ribosomal, 16S) Bacteremia/diagnosis/microbiology Bacteria/*genetics/isolation & purification/pathogenicity Bacterial Infections/diagnosis/microbiology Base Sequence Cerebrospinal Fluid/microbiology DNA Primers/genetics DNA Probes/genetics Genes, Bacterial Gram-Negative Bacteria/genetics Gram-Positive Bacteria/genetics Human Meningitis, Bacterial/diagnosis/microbiology Molecular Sequence Data Nucleic Acid Hybridization *Polymerase Chain Reaction/statistics & numerical data RNA, Bacterial/*genetics RNA, Ribosomal, 16S/*genetics Sensitivity and Specificity Sequence Homology, Nucleic Acid Species Specificity
Remarks: A set of broad-range PCR primers for the 16S rRNA gene in bacteria were
URL: 94201356
Data: (ATCC 10556) Type strain / ATCC in 1949 / Subacute bacterial endocarditis / Group H, Type I / Hare, R. (1935) J. path. Bact. 41, 499 / White, J. C. & Niven, C. F. Jr. (1946) J. Bact. 51, 717 / Washburn, M. R. et al. (1946) J. Bact. 51, 723
Accession Date: 01/01/1949
History: ATCC ,WASHINGTON D C ,1949
Authority: White and Niven 1946
Depositor: ATCC
Taxonomy: TaxLink: S2866 (Streptococcus sanguinis white and niven 1946) - Date of change: 16/06/2007 by NCTCUp to 16/06/2007: ? (NCTC 7863) - Date of change: 04/02/2003
Biosafety Responsibility: It is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country

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